| Product SKU | BCACP-000653 |
| Product Description | Recombinant Human Fibroblast growth factor 23 (FGF23)(R179Q) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 25-251aa(R179Q). [Accession | Q9GZV9]. |
| Uniprot No. | Q9GZV9 |
| Gene Names | FGF23 |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Expression System | Mammalian cell |
| Expression Region | 25-251aa(R179Q) |
| Species | Homo sapiens (Human) |
| Tag Info | C-terminal 10xHis-tagged |
| Molecular weight | 31.8 kDa |
| Activity | Measured by its binding ability in a functional ELISA. Immobilized Mouse Il21r at 2 μg/mL can bind Mouse Il21 . The EC50 is 6.858-8.187 ng/mL.;Mouse Il21 captured on Protein A Chip can bind Recombinant Mouse Il21r with an affinity constant of 0.146 nM as detected by MetaSPR Assay (WeSPRTM 200). |
| Buffer | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Alternative Names | Fibroblast growth factor 23; FGF-23; Phosphatonin; Tumor-derived hypophosphatemia-inducing factor [Cleaved into: Fibroblast growth factor 23 N-terminal peptide; Fibroblast growth factor 23 C-terminal peptide] |
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.
