Protein Expression Service
BioCrest Sci provides an end-to-end protein expression service for custom recombinant protein production, from sequence review and construct design through expression, purification, and quality control. With 12,000+ recombinant proteins delivered and a 90% project success rate, our recombinant protein expression services support challenging targets including transmembrane proteins, kinases, enzymes, and viral antigens for drug discovery, antibody development, diagnostics, structural biology, and functional assays.
Protein Expression Service Scope and Deliverables
BioCrest Sci's custom protein expression service covers sequence review, codon optimization, construct and tag design, gene synthesis and cloning, small-scale expression screening, scale-up, protein purification, buffer exchange, and project-specific quality control. Depending on project scope, deliverables can include purified recombinant protein, expression and purification data, SDS-PAGE results, concentration measurements, endotoxin results, and optional activity or binding data.
Why BioCrest Sci
Protein Expression Systems
This page is the parent comparison hub for BioCrest Sci protein expression services. Use the system-specific pages for detailed workflows and requirements: bacterial / E. coli expression, mammalian expression, yeast expression, insect baculovirus expression, cell-free expression, and transmembrane protein expression.
✔ Fastest (3–5 weeks)
✔ Low cost, high yield
⚠ No glycosylation, possible solubility issues
✔ Eukaryotic folding, moderate glycosylation
✔ Gram‑scale production
⚠ Longer optimization for secreted proteins
✔ Scalable, high safety
✔ Suitable for multimeric & some membrane proteins
⚠ Glycosylation differs from mammals
✔ Native conformation, full glycosylation
✔ Secreted expression, easy purification
⚠ Higher cost, longer timeline
✔ Full-length membrane proteins (GPCRs, ion channels, transporters)
✔ Multiple display platforms available
✔ Native or near-native conformation
✔ Rapid protein synthesis without live cells
✔ Ideal for toxic or membrane proteins
✔ High-throughput screening compatible
Transmembrane Protein Display Platforms
VLP Platform
Displayed Transmembrane Proteins
Virus-like particle platform for membrane protein presentation in native-like environment
Detergent Platform
Displayed Transmembrane Proteins
Detergent-solubilized membrane proteins for biochemical and structural studies
Synthetic Nanodisc Platform
Displayed Transmembrane Proteins
Synthetic nanodisc-embedded membrane proteins for maximal stability and functionality
Expression System Selection Guide
| Primary Priority | Recombinant System | Turnaround Time | Relative Cost | Key Limitation | Main Applications |
|---|---|---|---|---|---|
| Fastest protein acquisition | Bacterial (E. coli) | 3–5 weeks | Low | No glycosylation; solubility | Antigens, cytosolic proteins, enzymes |
| Native conformation & glycosylation | Mammalian | 6–8 weeks | High | Lower expression yield | Therapeutic targets, functional studies, antibodies |
| Membrane proteins / VLPs | Insect (Baculovirus) | 6–8 weeks | Medium | Scale‑up complexity | Vaccine development, structural studies |
| Full-length transmembrane proteins | VLP / Detergent / Nanodisc / Cell-Free | 4–8 weeks | Medium-High | Platform selection critical | GPCRs, ion channels, transporters, functional analysis |
| Toxic or difficult proteins | Cell-Free | 2–4 weeks | Medium | Limited for large-scale | Toxic proteins, membrane proteins, rapid screening |
Service Process
Frequently Asked Questions
Featured Success
Mammalian system + Nanodisc platform → high-purity, ligand binding confirmed.
Insect system → 50+ mg/L, used in ELISA kits worldwide.
Cell-free system → rapid expression, active conformation achieved.
Mammalian expression + VLP display → functional for antibody screening.
Catalog Recombinant Proteins
Need a ready-to-use research reagent instead of a custom project? Explore BioCrest Sci recombinant protein products by protein family, species, expression system, tag, and application.
Email: protein@biocrest.com | Fast response within 24h
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