NON-GMP RESEARCH SERVICE
Mammalian Cell Protein Expression Service
BioCrestSci provides non-GMP mammalian cell protein expression for research targets that may benefit from eukaryotic folding, secretion, disulfide-bond formation or mammalian post-translational processing. The service connects construct planning, transient expression, small-scale production, basic purification and defined quality-control reporting.
A DECISION-READY WORKFLOW
Why Choose BioCrestSci
Target-aware construct and host planning
Sequence boundaries, signal peptides, tag position, secretion strategy and host-cell compatibility are considered before transient expression begins.
Milestone-based experimental review
Transfection, expression analysis, production, purification and QC are separated into reviewable stages so later work can reflect the evidence generated earlier.
Purification included in the standard package
The $1,440 package includes basic protein purification. Polishing, endotoxin control and bioactivity work are added only when the target and intended use require them.
FROM CONSTRUCT TO CHARACTERIZATION
Six-Stage Mammalian Cell Protein Expression Workflow
SCOPE, DELIVERABLES AND PRICING
Stage 01–06 Technical Details
The standard package covers Stages 02–05. Stage 01 gene synthesis and Stage 06 options are separate. Final scope is confirmed after technical assessment.
Gene Synthesis and Vector Construction
Description
Prepare a sequence-verified mammalian expression construct with an agreed signal peptide and affinity or fusion tag.
Key Considerations
- Coding-region accuracy
- reading frame
- signal peptide
- promoter and vector compatibility
- tag location
- secretion versus intracellular expression.
Deliverables
Expression-ready construct and sequence or vector documentation.
Price
$1.55/aa, charged separately
Mammalian Transient Transfection
Description
Introduce the construct into a suitable mammalian host and establish the agreed transient-transfection conditions.
Key Considerations
- Host selection
- transfection format
- cell viability
- secretion
- basal expression
- appropriate positive and negative controls.
Deliverables
Transfected culture material and a documented transfection record.
Price
$800
Small Scale Expression
Description
Evaluate expression and recoverability under an agreed culture and harvest plan.
Key Considerations
- Expression signal
- estimated titer where measurable
- culture duration
- harvest timing
- intracellular versus secreted recovery
- sample stability.
Deliverables
Small-scale expression result and selected production condition.
Price
$320
Basic Protein Purification
Description
Apply a target-compatible basic affinity-purification workflow and prepare the sample for analytical review.
Key Considerations
- Tag accessibility
- harvest matrix
- resin compatibility
- aggregation
- proteolysis
- target buffer and recovery.
Deliverables
Basic purified protein fraction and recorded purification conditions.
Price
Included in the $1,440 package; no separate basic purification fee
Basic Quality Control and Reporting
Description
Assess the recovered protein with native PAGE, reducing SDS-PAGE and a standard COA or study report.
Key Considerations
- Apparent molecular mass
- oligomeric behavior
- concentration
- sample stability
- acceptance criteria matched to intended use.
Deliverables
Native PAGE, reducing SDS-PAGE and standard COA or study report.
Price
$320 subtotal: Native PAGE $80; reducing SDS-PAGE $80; report $160
Optional Characterization and Process Add Ons
Description
Add target-specific polishing, endotoxin-control or functional work after reviewing protein recovery and assay requirements.
Key Considerations
- Protein stability after added processing
- analytical method suitability
- assay controls
- numerical acceptance criteria
- recovery tradeoffs.
Deliverables
Optional Endotoxin Removal, quotation upon request, target <0.1 EU/µg; Bioactivity Measurement, quoted by assay scope; SEC polishing, buffer exchange or tag cleavage when appropriate.
Price
Quoted separately; <0.1 EU/µg is a target and is not guaranteed
TARGET-SPECIFIC RISK REVIEW
Mammalian Expression Optimization
RESEARCH APPLICATIONS
Designed for Diverse Recombinant Protein Programs
- Cytokines and growth factors
- Receptors and Fc fusions
- Antibody research
- Binding and interaction studies
- Structural biology
- Diagnostic reagent research
DEFINED BASIC QC
Quality Control and Project Reporting
Basic QC includes native PAGE, reducing SDS-PAGE and a standard COA or study report. Additional analytical work is selected according to the molecule and intended use.
PROJECT PLANNING
Frequently Asked Questions
Which proteins are suitable for mammalian cell expression?
Secreted, disulfide-rich, glycosylated and multidomain proteins are common candidates, including cytokines, receptors, Fc fusions and selected antibody formats. Suitability depends on the sequence, required post-translational processing and intended experiment.
What is included in the standard mammalian package?
The $1,440 standard package includes transient transfection, small-scale expression, basic protein purification, native PAGE, reducing SDS-PAGE and a standard COA or study report. Gene synthesis and optional Stage 06 work are separate.
Can I provide my own mammalian expression plasmid?
A customer-supplied construct can be assessed when the vector map, insert sequence, resistance marker, promoter, signal peptide, tag configuration and available expression history are provided. Verification or adaptation work is agreed before production.
What happens if expression or secretion is low?
The project can be reviewed for construct boundaries, signal peptide, host choice, transfection conditions, culture duration and harvest strategy. Additional optimization is separately scoped, and a specific yield cannot be promised before testing.
Can you provide low endotoxin or bioactive mammalian protein?
Optional endotoxin removal and bioactivity measurement can be discussed. The <0.1 EU/µg value is a project target rather than a universal guarantee, and bioactivity work is quoted according to assay design and controls.
START WITH A TECHNICAL REVIEW
Discuss Your Mammalian Cell Protein Expression Project
Share the target sequence, preferred construct, intended application and requested analytical scope. BioCrestSci will assess feasibility and define a project-specific plan.