Custom Antibody Production Services

BioCrest Sci · Custom Antibody Production Service

End‑to‑End Antibody Development · Multi‑platform Customization · Transparent Science

Scientist‑to‑Scientist

BioCrest Sci provides end-to-end custom antibody production services for research, diagnostic, and therapeutic discovery projects. We develop custom-made antibodies against recombinant proteins, peptides, membrane proteins, GPCRs, viral and bacterial antigens, haptens, and other difficult targets. Projects can include antigen design, host selection, immunization or library screening, clone selection, purification, sequencing, application-specific validation, and quality control.

Antibody Customization Services

Select the service that matches your project needs. Each option leads to a dedicated service page with detailed workflows, timelines, and technical specifications.

Custom Monoclonal Antibody

3–6 months

High‑specificity antibodies from a single clone. Ideal for diagnostic development, flow cytometry, IHC, and long‑term reproducibility.

Diagnostics Flow IHC

Custom Polyclonal Antibody

2–4 months

Rapid generation of antibody populations recognizing multiple epitopes. Well‑suited for WB, ELISA, protein tracking, and exploratory studies.

WB ELISA Exploration

Custom Recombinant Antibody

4–10 weeks (after sequence)

Sequence‑defined, renewable antibodies with batch‑to‑batch consistency. Supports engineering, humanization, affinity maturation & scalable manufacturing.

Therapeutics Engineering

Custom Nanobody (VHH)

3–5 months

Single‑domain antibody fragments with small size and high stability. Perfect for intracellular targeting, structural biology, CAR‑T, and biosensors.

Imaging CAR Intracellular

Neutralizing Antibody

Function‑driven

Functionally validated antibodies that block biological activity — viral entry, receptor‑ligand interaction, cytokine signaling, or enzymatic inhibition.

Antiviral Blocking

Comparison of Antibody Production Platforms

Select the right platform based on your research needs.

Service Type	Main Advantages	Typical Timeline	Best‑Fit Applications
Polyclonal Antibody	Fast and broad epitope recognition	~2–4 months	WB, ELISA, exploratory studies
Monoclonal Antibody	Specificity and reproducibility	~3–6 months	Diagnostics, flow cytometry, IHC
Recombinant Antibody	Sequence‑defined renewable reagent	~1–3 months after sequence	Therapeutic and engineered antibody research
Nanobody (VHH)	Small size and high stability	~3–5 months	Imaging, intracellular targeting, CAR research
Neutralizing Antibody	Functional biological inhibition	Variable	Antiviral and blocking studies

📌 Actual timelines vary depending on antigen immunogenicity, screening complexity & validation requirements.

Custom Antibody Production Workflow

Target and application review: define the antigen, intended assay, species reactivity, format, quantity, and validation requirements.
Antigen and immunogen design: select recombinant protein, peptide, cell-based, membrane-protein, or other antigen strategies.
Host or discovery-platform selection: choose rabbit, mouse, rat, goat, chicken, llama/VHH, hybridoma, single B-cell, or phage-display workflows according to the project.
Immunization or library screening: monitor immune response or enrichment and identify antigen-specific candidates.
Clone screening and validation: evaluate binding, specificity, cross-reactivity, affinity, and performance in the intended application.
Production, purification, and delivery: scale selected antibodies and provide the agreed antibody material, data, and QC documentation.

Host Species and Discovery Platforms

Host or Platform	Typical Use	Key Advantage
Rabbit	Peptides, recombinant proteins, phospho-specific and low-immunogenicity targets	Strong affinity and broad research applicability
Mouse or Rat	Monoclonal antibody development, screening, and diagnostic research	Established hybridoma and low-antigen workflows
Goat, Sheep, or Chicken	Higher-yield polyclonal production, conserved mammalian targets, and IgY	Scale or phylogenetic-distance advantages
Llama / VHH	Nanobody discovery, structural biology, imaging, and difficult epitopes	Small, stable single-domain antibodies
Single B-Cell or Phage Display	Rapid sequence-defined discovery, fully human or difficult targets	Direct sequence recovery and recombinant production

Project Deliverables and Quality Control

Deliverables are defined by project scope and may include purified antibody, immune serum, positive hybridoma clones, antibody gene sequences, recombinant antibody samples, isotyping results, raw screening data, and application-specific validation reports. Quality-control options include SDS-PAGE, concentration measurement, endotoxin testing, binding ELISA, Western blot, flow cytometry, IHC/IF, SPR/BLI affinity analysis, neutralization, and cell-based functional assays.

Antigen Types Supported

Recombinant proteins, peptides (including phosphorylated), membrane proteins, GPCRs, ion channels, viral/bacterial antigens, toxins, haptens, and more.

Recombinant proteins Phospho‑peptides Membrane proteins GPCRs Viral antigens Fusion proteins Cytokines Low immunogenicity targets

Our scientists assist with epitope selection, antigen design, and immunization optimization.

Screening & Validation Options

Customizable screening aligned with your intended application:

ELISA / MSD binding
Western blot
Flow cytometry
Immunofluorescence (IF/ICC)
Immunohistochemistry (IHC)
Neutralization assays
SPR / BLI affinity
Cell‑based functional assays
Pair screening for sandwich ELISA

📊 Antigen Integrity · Pre‑immunization QC & serum titer monitoring 🧪 Clone Stability · Monoclonal screening & contamination testing 📝 Sequence Verification · For recombinant antibodies + SDS‑PAGE, endotoxin, concentration QC 🎯 Application‑Specific Validation · Tailored to your downstream assay, not generic testing

💡 If a strategy is unlikely to succeed, we will tell you upfront and recommend an alternative approach.

Why Researchers Choose BioCrest

Multiple platforms — monoclonal, polyclonal, recombinant, nanobody, neutralizing all in one place

Difficult target expertise — membrane proteins, conformational epitopes, low‑immunogenicity antigens

Transparent communication — weekly updates, raw data access, honest feasibility assessment

Flexible project scope — from exploratory generation to engineered functional antibodies

Frequently Asked Questions

❓ Which antibody platform is best for my project?

Depends on your antigen, timeline, downstream application, and whether you need recombinant or functional antibodies. Our scientists offer free strategy consultation.

❓ Can you generate antibodies against membrane proteins / GPCRs?

Yes. Peptide immunization, recombinant extracellular domains, cell‑based immunization, and recombinant screening workflows are available.

❓ Do you offer antibody sequencing and recombinant recovery?

Yes. Integrated into monoclonal, nanobody, and recombinant antibody workflows.

❓ What is included in a custom antibody production project?

A project can include antigen design, host or platform selection, immunization or library screening, clone selection, purification, sequencing, and validation. The final workflow and deliverables are defined around the intended application.

❓ Can BioCrest develop custom-made antibodies for a specific assay?

Yes. Screening and validation can be aligned with Western blot, ELISA, flow cytometry, IHC, IF/ICC, immunoprecipitation, neutralization, affinity measurement, or cell-based functional assays.

Start Your Custom Antibody Project

Contact Our Scientists → No obligation · Scientist‑to‑scientist conversation