Recombinant Bovine Adrenodoxin, mitochondrial (FDX1), Bos taurus (Bovine), E.coli, N-terminal 10xHis-tagged, 19.5kDa, purity >85% as determined by SDS-PAGE.

Recombinant Bovine Adrenodoxin, mitochondrial (FDX1)

Recombinant Bovine Adrenodoxin, mitochondrial (FDX1) — Essential for the synthesis of various steroid hormones. Purity >85%.

SKU: BCRECP-002718 Category:
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Product Specifications

Product SkuBCRECP-002718
Product DescriptionRecombinant Bovine Adrenodoxin, mitochondrial (FDX1) Protein is expressed from E.coli with N-terminal 10xHis-tagged. It contains 59-186aa. [Accession | P00257].
Uniprot No.P00257
Gene NamesFDX1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region59-186aa
SpeciesBos taurus (Bovine)
Tag InfoN-terminal 10xHis-tagged
Molecular weight19.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMetabolism

Function

Essential for the synthesis of various steroid hormones. Participates in the reduction of mitochondrial cytochrome P450 for steroidogenesis. Transfers electrons from adrenodoxin reductase to CYP11A1, a cytochrome P450 that catalyzes cholesterol side-chain cleavage to produce pregnenolone, the precursor of most steroid hormones.

Biological Context

Subcellular Location: Mitochondrion matrix
Tissue Specificity: Detected in adrenal cortex and corpus luteum (at protein level)

Product Specifications

Recombinant Bovine Adrenodoxin, mitochondrial (FDX1) is a recombinant protein from Bos taurus (Bovine), expressed in E.coli, covering amino acids 59-186aa, with N-terminal 10xHis-tagged tag, molecular weight 19.5kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Essential for the synthesis of various steroid hormones. Participates in the reduction of mitochondrial cytochrome P450 for steroidogenesis. Transfers electrons from adrenodoxin reductase to CYP11A1, a cytochrome P450 that catalyzes cholesterol side-chain cleavage to produce pregnenolone, the precursor of most steroid hormones. Does not form a ternary complex with adrenodoxin reductase and CYP11A1 but shuttles between the two enzymes to transfer electrons

Subcellular Location

Mitochondrion matrix

Tissue Specificity

Detected in adrenal cortex and corpus luteum (at protein level)

Subunit

Interacts with CYP11A1

Gene: FDX1  |  Organism: Bos taurus  |  Synonyms: Adrenal ferredoxin; Ferredoxin-1; Hepato-ferredoxin
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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