Recombinant Mycobacterium tuberculosis Glutamine synthetase (glnA1), Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 60.9kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mycobacterium tuberculosis Glutamine synthetase (glnA1)

Recombinant Mycobacterium tuberculosis Glutamine synthetase (glnA1) — Involved in nitrogen metabolism via ammonium assimilation. Purity >90%.

SKU: BCRECP-002869 Category:
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Product Specifications

Product SkuBCRECP-002869
Product DescriptionRecombinant Mycobacterium tuberculosis Glutamine synthetase (glnA1) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 2-478aa. [Accession | P9WN39].
Uniprot No.P9WN39
Gene NamesglnA1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-478aa
SpeciesMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight60.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Involved in nitrogen metabolism via ammonium assimilation. Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia. Also able to use GTP.

Biological Context

Subcellular Location: Cytoplasm

Product Specifications

Recombinant Mycobacterium tuberculosis Glutamine synthetase (glnA1) is a recombinant protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv), expressed in E.coli, covering amino acids 2-478aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 60.9kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Involved in nitrogen metabolism via ammonium assimilation. Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia (PubMed:12819079, PubMed:7937767). Also able to use GTP (PubMed:7937767). D-glutamate is a poor substrate, and DL-glutamate shows about 50% of the standard specific activity (PubMed:7937767). Also plays a key role in controlling the ammonia levels within infected host cells and so contributes to the pathogens capacity to inhibit phagosome acidification and phagosome-lysosome fusion (PubMed:12819079, PubMed:7937767). Involved in cell wall biosynthesis via the production of the major component poly-L-glutamine (PLG) (PubMed:10618433, PubMed:7937767). PLG synthesis in the cell wall occurs only in nitrogen limiting conditions and on the contrary high nitrogen conditions inhibit PLG synthesis (Probable)

Subcellular Location

Cytoplasm

Subunit

Oligomer of 12 subunits arranged in the form of two hexagons

Gene: glnA1  |  Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)  |  Synonyms: Glutamate--ammonia ligase; Glutamine synthetase I beta
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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