Mouse anti-Human/Mouse/Rat CD5 Monoclonal Antibody

Function

Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis. Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL (PubMed:1384049, PubMed:1385158). Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation (PubMed:23376399). Modulates B-cell biology through ERK1/2 activation in a Ca(2+)-dependent pathway via the non-selective Ca(2+) channel TRPC1, leading to IL-10 production (PubMed:27499044)

Subcellular Location

Cell membrane (Single-pass type I membrane protein)

Subunit

Interacts with CD72/LYB-2 (PubMed:1711157). Interacts with PTPN6/SHP-1 (By similarity). Interacts with CBL (PubMed:23376399). Interacts with CD5L (By similarity). Interacts with CD3Z/CD247 (PubMed:1384049, PubMed:1385158)

• Jones N.H. et al. Isolation of complementary DNA clones encoding the human lymphocyte glycoprotein T1/Leu-1. — Nature (1986)
• Calvo J. et al. Evolutionarily conserved transcription regulatory elements within the 5'-flanking region of the human CD5 gene. — Tissue Antigens (1996)
• Livingston R.J. et al. PubMed: CI-JGR3DF5COMGD (OCT-)
• Ota T. et al. Complete sequencing and characterization of 21,243 full-length human cDNAs. — Nat. Genet. (2004)
• Taylor T.D. et al. Human chromosome 11 DNA sequence and analysis including novel gene identification. — Nature (2006)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)
• van de Velde H. et al. The B-cell surface protein CD72/Lyb-2 is the ligand for CD5. — Nature (1991)
• Osman N. et al. Evidence for an association between the T cell receptor/CD3 antigen complex and the CD5 antigen in human T lymphocytes. — Eur. J. Immunol. (1992)