Mouse anti-Human/Rat sapiens (Human) DNAJB1 Monoclonal Antibody
Mouse anti-Human/Rat sapiens (Human) DNAJB1 Monoclonal Antibody - Image 2

Mouse anti-Human/Rat sapiens (Human) DNAJB1 Monoclonal Antibody

Mouse anti-Human/Rat sapiens (Human) DNAJB1 Monoclonal Antibody — Interacts with HSP70 and can stimulate its ATPase activity.

SKU: BCREC-000494MA Category:
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Product Specifications

Uniprot No.P25685
Target NamesDNAJB1
Species ReactivityMouse
ImmunogenRecombinant Human DNAJB1 protein
Immunogen SpeciesHomo sapiens (Human)
ConjugateNon-conjugated
IsotypeMouse IgG2b
ClonalityMonoclonal
ApplicationsELISA, WB, FC
BufferPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Function

Interacts with HSP70 and can stimulate its ATPase activity. Acts also with TTC1 as a chaperone adapter that regulates HSP70-dependent folding process by interacting with the HSP70 amino terminal region. Stimulates the association between HSC70 and HIP.

Biological Context

Subcellular Location: Cytoplasm; Nucleus; Nucleus, nucleolus

Product Specifications

Mouse anti-Human/Rat sapiens (Human) DNAJB1 Monoclonal Antibody is a recombinant protein. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Interacts with HSP70 and can stimulate its ATPase activity. Acts also with TTC1 as a chaperone adapter that regulates HSP70-dependent folding process by interacting with the HSP70 amino terminal region (PubMed:14503850). Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response (PubMed:9499401). Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro) (PubMed:24318877)

Subcellular Location

Cytoplasm; Nucleus; Nucleus, nucleolus

Subunit

Interacts with DNAJC3 (PubMed:9920933). Interacts with HSF1 (via transactivation domain); this interaction results in the inhibition of heat shock- and HSF1-induced transcriptional activity during the attenuation and recovery phase period of the heat shock response (PubMed:9499401). Interacts with BAG3 (PubMed:30559338). Interacts with TTC1 and HSP70/HSPA1A (PubMed:14503850)

Gene: DNAJB1  |  Organism: Homo sapiens  |  Synonyms: DnaJ protein homolog 1; Heat shock 40 kDa protein 1; Human DnaJ protein 1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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