Recombinant Dog B-lymphocyte antigen CD20 (MS4A1)-VLPs (Active), Canis lupus familiaris (Dog) (Canis familiaris), Mammalian cell, C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions), 34.4 kDa, purity >90% as determined by SEC-HPLC.
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Recombinant Dog B-lymphocyte antigen CD20 (MS4A1)-VLPs (Active)

Recombinant Dog B-lymphocyte antigen CD20 (MS4A1)-VLPs (Active) — B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Purity >90%.

SKU: BCACP-000198 Categories: , ,
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Product SKUBCACP-000198
Product DescriptionRecombinant Canis familiaris B-lymphocyte antigen CD20 (MS4A1)-VLPs protein is expressed from Mammalian cell with C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions). It contains 1-297aa. [Accession | Q3C2E2].
Uniprot No.Q3C2E2
Gene NamesMS4A1
PurityGreater than 90% as determined by SEC-HPLC.
Expression SystemMammalian cell
Expression Region1-297aa
SpeciesCanis lupus familiaris (Dog) (Canis familiaris)
Tag InfoC-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)
Molecular weight34.4 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis CD44 at 2 μg/mL can bind Anti-CD44 monoclonal antibody , the EC50 is 64.18-90.91 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative Names(Membrane-spanning 4-domains subfamily A member 1)(CD antigen CD20)

Function

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein); Cell membrane (Lipid-anchor)
Tissue Specificity: Expressed in PBMCs and lymph node from healthy dogs, in B-cells of canine lymphoma, but not in T-cell lymphoma cells and non-T and non-B-cell lymphoma cells

Product Specifications

Recombinant Dog B-lymphocyte antigen CD20 (MS4A1)-VLPs (Active) is a recombinant protein from Canis lupus familiaris (Dog) (Canis familiaris), expressed in Mammalian cell, covering amino acids 1-297aa, with C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions) tag, molecular weight 34.4 kDa, purity Greater than 90% as determined by SEC-HPLC.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR

Subcellular Location

Cell membrane (Multi-pass membrane protein); Cell membrane (Lipid-anchor)

Tissue Specificity

Expressed in PBMCs and lymph node from healthy dogs, in B-cells of canine lymphoma, but not in T-cell lymphoma cells and non-T and non-B-cell lymphoma cells

Subunit

Forms homotetramers. Interacts with the heavy and light chains of cell surface IgM, the antigen-binding components of the BCR

Gene: MS4A1  |  Organism: Canis lupus familiaris  |  Synonyms: Membrane-spanning 4-domains subfamily A member 1

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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