Recombinant Human C-type lectin domain family 5 member A (CLEC5A), partial, Biotinylated (Active), Homo sapiens (Human), Mammalian cell, N-terminal 10xHis-Avi-tagged, 26.7 kDa, purity >95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.
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Recombinant Human C-type lectin domain family 5 member A (CLEC5A), partial, Biotinylated (Active)

Recombinant Human C-type lectin domain family 5 member A (CLEC5A), partial, Biotinylated (Active) — (Microbial infection) Critical macrophage receptor for dengue virus serotypes 1-4. Purity >95%.

SKU: BCACP-000652 Categories: ,
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Product SKUBCACP-000652
Product DescriptionRecombinant Human C-type lectin domain family 5 member A (CLEC5A), Biotinylated protein is expressed from Mammalian cell with N-terminal 10xHis-Avi-tagged. It contains 28-188aa. [Accession | Q9NY25].
Uniprot No.Q9NY25
Gene NamesCLEC5A
PurityGreater than 95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.
Expression SystemMammalian cell
Expression Region28-188aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-Avi-tagged
Molecular weight26.7 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human CXCL3 at 2 μg/mL can bind Anti-CXCL3 recombinant antibody . The EC50 is 9.702-11.20 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesC-type lectin domain family 5 member A; C-type lectin superfamily member 5; Myeloid DAP12-associating lectin 1; MDL-1

Function

(Microbial infection) Critical macrophage receptor for dengue virus serotypes 1-4. The binding of dengue virus to CLEC5A triggers signaling through the phosphorylation of TYROBP. This interaction does not result in viral entry, but stimulates pro-inflammatory cytokine release.

Biological Context

Subcellular Location: Cell membrane (Single-pass type II membrane protein)
Tissue Specificity: Highly expressed in bone marrow with lower levels in synovium, lung and bronchus. Expressed in peripheral blood monocytes and in the monocyte/macrophage cell lines U-937 and Mono-Mac-6, but not in cell lines of other origins. Expression is down-regulated when monocytes differentiate into dendritic cells

Product Specifications

Recombinant Human C-type lectin domain family 5 member A (CLEC5A), partial, Biotinylated (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 28-188aa, with N-terminal 10xHis-Avi-tagged tag, molecular weight 26.7 kDa, purity Greater than 95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

(Microbial infection) Critical macrophage receptor for dengue virus serotypes 1-4 (PubMed:18496526, PubMed:21566123). The binding of dengue virus to CLEC5A triggers signaling through the phosphorylation of TYROBP (PubMed:18496526). This interaction does not result in viral entry, but stimulates pro-inflammatory cytokine release (PubMed:18496526)

Subcellular Location

Cell membrane (Single-pass type II membrane protein)

Tissue Specificity

Highly expressed in bone marrow with lower levels in synovium, lung and bronchus (PubMed:20212065). Expressed in peripheral blood monocytes and in the monocyte/macrophage cell lines U-937 and Mono-Mac-6, but not in cell lines of other origins (PubMed:10449773). Expression is down-regulated when monocytes differentiate into dendritic cells (PubMed:10449773)

Subunit

(Microbial infection) Interacts with dengue virus envelope protein E (PubMed:18496526, PubMed:21566123)

Gene: CLEC5A  |  Organism: Homo sapiens  |  Synonyms: C-type lectin superfamily member 5; Myeloid DAP12-associating lectin 1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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