Recombinant Human GTP:AMP phosphotransferase, mitochondrial (AK3), Homo sapiens (Human), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 32.6kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human GTP:AMP phosphotransferase, mitochondrial (AK3)

Recombinant Human GTP:AMP phosphotransferase, mitochondrial (AK3) — Mitochondrial adenylate kinase with a specific GTP:AMP phosphotransferase activity. Purity >90%.

SKU: BCRECP-000051 Categories: ,
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Product Specifications

Product SkuBCRECP-000051
Product DescriptionRecombinant Human GTP:AMP phosphotransferase, mitochondrial (AK3) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-227aa. [Accession | Q9UIJ7].
Uniprot No.Q9UIJ7
Gene NamesAK3
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-227aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight32.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMetabolism

Function

Mitochondrial adenylate kinase with a specific GTP:AMP phosphotransferase activity. Could also use ITP as phosphate donor. Its physiological function is to recycle GTP into GDP which is necessary for the TCA cycle in the mitochondrial matrix (Probable).

Biological Context

Subcellular Location: Mitochondrion matrix
Tissue Specificity: Highly expressed in heart, skeletal muscle and liver, moderately expressed in pancreas and kidney, and weakly expressed in placenta, brain and lung

Product Specifications

Recombinant Human GTP:AMP phosphotransferase, mitochondrial (AK3) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-227aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 32.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Mitochondrial adenylate kinase with a specific GTP:AMP phosphotransferase activity (PubMed:11485571, PubMed:32822537). Could also use ITP as phosphate donor (PubMed:11485571). Its physiological function is to recycle GTP into GDP which is necessary for the TCA cycle in the mitochondrial matrix (Probable)

Subcellular Location

Mitochondrion matrix

Tissue Specificity

Highly expressed in heart, skeletal muscle and liver, moderately expressed in pancreas and kidney, and weakly expressed in placenta, brain and lung

Subunit

Monomer

Gene: AK3  |  Organism: Homo sapiens  |  Synonyms: Adenylate kinase 3; Adenylate kinase 3 alpha-like 1; Adenylate kinase isozyme 3
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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