Recombinant Human Neuroglobin (NGB), Homo sapiens (Human), E.coli, N-terminal 6xHis-SUMO-tagged, 32.9kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Neuroglobin (NGB)

Recombinant Human Neuroglobin (NGB) — Monomeric globin with a bis-histidyl six-coordinate heme-iron atom through which it can bind dioxygen, carbon monoxide and nitric oxide. Purity >90%.

SKU: BCRECP-001240 Category:
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Product Specifications

Product SkuBCRECP-001240
Product DescriptionRecombinant Human Neuroglobin (NGB) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 1-151aa. [Accession | Q9NPG2].
Uniprot No.Q9NPG2
Gene NamesNGB
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-151aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight32.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasTransport

Function

Monomeric globin with a bis-histidyl six-coordinate heme-iron atom through which it can bind dioxygen, carbon monoxide and nitric oxide. Could help transport oxygen and increase its availability to the metabolically active neuronal tissues, though its low quantity in tissues as well as its high affinity for dioxygen, which may limit its oxygen-releasing ability, argue against it. The ferrous/deoxygenated form exhibits a nitrite reductase activity and it could produce nitric oxide which in turn inhibits cellular respiration in response to hypoxia.

Biological Context

Subcellular Location: Cytoplasm, cytosol; Mitochondrion matrix
Tissue Specificity: Predominantly expressed in brain, the strongest expression is seen in the frontal lobe, the subthalamic nucleus and the thalamus

Product Specifications

Recombinant Human Neuroglobin (NGB) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-151aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 32.9kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Monomeric globin with a bis-histidyl six-coordinate heme-iron atom through which it can bind dioxygen, carbon monoxide and nitric oxide (PubMed:11473128, PubMed:12962627, PubMed:24699645). Could help transport oxygen and increase its availability to the metabolically active neuronal tissues, though its low quantity in tissues as well as its high affinity for dioxygen, which may limit its oxygen-releasing ability, argue against it (PubMed:11473128, PubMed:12860983, PubMed:12962627, PubMed:24699645). The ferrous/deoxygenated form exhibits a nitrite reductase activity and it could produce nitric oxide which in turn inhibits cellular respiration in response to hypoxia (PubMed:21296891). In its ferrous/deoxygenated state, it may also exhibit GDI (Guanine nucleotide Dissociation Inhibitor) activity toward heterotrimeric G-alpha proteins, thereby regulating signal transduction to facilitate neuroprotective responses in the wake of hypoxia and associated oxidative stress (PubMed:12860983, PubMed:18302932)

Subcellular Location

Cytoplasm, cytosol; Mitochondrion matrix

Tissue Specificity

Predominantly expressed in brain, the strongest expression is seen in the frontal lobe, the subthalamic nucleus and the thalamus

Subunit

Monomer (PubMed:11029004). Homodimer and homotetramer; disulfide-linked. Mainly monomeric but also detected as part of homodimers and homotetramers (By similarity). Interacts with 14-3-3 proteins; regulates the phosphorylation of NGB (PubMed:21965683). Could interact (ferrous form) with G-alpha(i) proteins (GTP-bound form) (PubMed:12860983)

Gene: NGB  |  Organism: Homo sapiens  |  Synonyms: Nitrite reductase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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