Recombinant Human Protein mono-ADP-ribosyltransferase PARP4 (PARP4), partial, Homo sapiens (Human), E.coli, N-terminal 10xHis-tagged, 55.0kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human Protein mono-ADP-ribosyltransferase PARP4 (PARP4), partial

Recombinant Human Protein mono-ADP-ribosyltransferase PARP4 (PARP4), partial — Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins. Purity >90%.

SKU: BCRECP-001338 Categories: ,
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Product Specifications

Product SkuBCRECP-001338
Product DescriptionRecombinant Human Protein mono-ADP-ribosyltransferase PARP4 (PARP4) Protein is expressed from E.coli with N-terminal 10xHis-tagged. It contains 607-1046aa. [Accession | Q9UKK3].
Uniprot No.Q9UKK3
Gene NamesPARP4
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region607-1046aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged
Molecular weight55.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins. Component of the vault particle, which is a massive ribonucleoprotein complex that may play a role in cargo transport and signal transduction.

Biological Context

Subcellular Location: Cytoplasm; Nucleus; Cytoplasm, cytoskeleton, spindle
Tissue Specificity: Widely expressed; the highest levels are in the kidney; also detected in heart, placenta, lung, liver, skeletal muscle, spleen, leukocytes and pancreas

Product Specifications

Recombinant Human Protein mono-ADP-ribosyltransferase PARP4 (PARP4), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 607-1046aa, with N-terminal 10xHis-tagged tag, molecular weight 55.0kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins (PubMed:25043379). Component of the vault particle, which is a massive ribonucleoprotein complex that may play a role in cargo transport and signal transduction (PubMed:40691181)

Subcellular Location

Cytoplasm; Nucleus; Cytoplasm, cytoskeleton, spindle

Tissue Specificity

Widely expressed; the highest levels are in the kidney; also detected in heart, placenta, lung, liver, skeletal muscle, spleen, leukocytes and pancreas

Subunit

Associates with MVP to form part of the vault particle (PubMed:40691181). The vault is a massive cage-like structure; it consists of a dimer of half-vaults, with each half-vault comprising 39 identical MVP subunits (PubMed:40691181). The two half-vaults assemble to form the complete vault, where minor vault components such as PARP4, TEP1 and vault RNAs (vRNAs) may bind to the interior (PubMed:40691181, PubMed:10477748). Interacts with MVP (via MVP repeats 3 and 4); each MVP subunit binds a single PARP4 molecule, allowing each vault to house up to 78 PARP4 molecules (PubMed:40691181). Interacts with TEP1 (PubMed:10551828, PubMed:15169895)

Gene: PARP4  |  Organism: Homo sapiens  |  Synonyms: 193 kDa vault protein; ADP-ribosyltransferase diphtheria toxin-like 4; PARP-related/IalphaI-related H5/proline-rich; Poly [ADP-ribose] polymerase 4; Vault poly(ADP-ribose) polymerase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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