Recombinant Human DNA-directed DNA/RNA polymerase mu (POLM), Homo sapiens (Human), E.coli, N-terminal 6xHis-tagged, 58.8kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human DNA-directed DNA/RNA polymerase mu (POLM)

Recombinant Human DNA-directed DNA/RNA polymerase mu (POLM) — Catalyzes template-directed nucleotide addition during gap filling at DNA double-strand breaks. Purity >85%.

SKU: BCRECP-001415 Category:
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Product Specifications

Product SkuBCRECP-001415
Product DescriptionRecombinant Human DNA-directed DNA/RNA polymerase mu (POLM) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-494aa. [Accession | Q9NP87].
Uniprot No.Q9NP87
Gene NamesPOLM
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-494aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight58.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Catalyzes template-directed nucleotide addition during gap filling at DNA double-strand breaks. Functions as a gap-filling polymerase involved in repair of DNA double-strand breaks by non-homologous end joining (NHEJ). Displays reduced sugar discrimination, incorporating deoxyribonucleotides and ribonucleotides with similar efficiency on DNA primer strands, and is able to polymerize nucleotides on RNA-terminated primer strands in vitro.

Biological Context

Subcellular Location: Nucleus
Tissue Specificity: Predominantly expressed in peripheral lymphoid tissues

Product Specifications

Recombinant Human DNA-directed DNA/RNA polymerase mu (POLM) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-494aa, with N-terminal 6xHis-tagged tag, molecular weight 58.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Catalyzes template-directed nucleotide addition during gap filling at DNA double-strand breaks (PubMed:10747040, PubMed:10982892, PubMed:12640116, PubMed:12888504, PubMed:17483519, PubMed:17915942, PubMed:26240373). Functions as a gap-filling polymerase involved in repair of DNA double-strand breaks by non-homologous end joining (NHEJ) (PubMed:12640116, PubMed:17915942, PubMed:26240373). Displays reduced sugar discrimination, incorporating deoxyribonucleotides and ribonucleotides with similar efficiency on DNA primer strands, and is able to polymerize nucleotides on RNA-terminated primer strands in vitro (PubMed:12640116, PubMed:12888504). Participates in immunoglobulin (Ig) light chain gene rearrangement in V(D)J recombination (By similarity)

Subcellular Location

Nucleus

Tissue Specificity

Predominantly expressed in peripheral lymphoid tissues

Gene: POLM  |  Organism: Homo sapiens  |  Synonyms: Terminal transferase

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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