Recombinant Human POU domain, class 2, transcription factor 3 (POU2F3), Homo sapiens (Human), E.coli, N-terminal 10xHis-tagged, 53.5kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human POU domain, class 2, transcription factor 3 (POU2F3)

Recombinant Human POU domain, class 2, transcription factor 3 (POU2F3) — Transcription factor that binds to the octamer motif (5′-ATTTGCAT-3′) and regulates cell type-specific differentiation pathways. Purity >90%.

SKU: BCRECP-001420 Category:
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Product Specifications

Product SkuBCRECP-001420
Product DescriptionRecombinant Human POU domain, class 2, transcription factor 3 (POU2F3) Protein is expressed from E.coli with N-terminal 10xHis-tagged. It contains 1-436aa. [Accession | Q9UKI9].
Uniprot No.Q9UKI9
Gene NamesPOU2F3
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-436aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged
Molecular weight53.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and regulates cell type-specific differentiation pathways. Involved in the regulation of keratinocytes differentiation. The POU2F3-POU2AF2/POU2AF3 complex drives the expression of tuft-cell-specific genes, a rare chemosensory cells that coordinate immune and neural functions within mucosal epithelial tissues.

Biological Context

Subcellular Location: Nucleus
Tissue Specificity: Specifically expressed in epidermis and cultured keratinocytes

Product Specifications

Recombinant Human POU domain, class 2, transcription factor 3 (POU2F3) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-436aa, with N-terminal 10xHis-tagged tag, molecular weight 53.5kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and regulates cell type-specific differentiation pathways. Involved in the regulation of keratinocytes differentiation (PubMed:11329378). The POU2F3-POU2AF2/POU2AF3 complex drives the expression of tuft-cell-specific genes, a rare chemosensory cells that coordinate immune and neural functions within mucosal epithelial tissues (PubMed:35576971)

Subcellular Location

Nucleus

Tissue Specificity

Specifically expressed in epidermis and cultured keratinocytes

Subunit

Interacts (via the POU domain) with POU2AF1 and POU2AF2 in a DNA-dependent manner; this interaction recruits POU2AF2 to chromatin and increases POU2F3 transactivation activity

Gene: POU2F3  |  Organism: Homo sapiens  |  Synonyms: Octamer-binding protein 11; Octamer-binding transcription factor 11; Transcription factor PLA-1; Transcription factor Skn-1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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