Recombinant Human Tyrosine-protein phosphatase non-receptor type 1 (PTPN1), partial, Homo sapiens (Human), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 44.8kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Human Tyrosine-protein phosphatase non-receptor type 1 (PTPN1), partial

Recombinant Human Tyrosine-protein phosphatase non-receptor type 1 (PTPN1), partial — Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Purity >85%.

SKU: BCRECP-001474 Categories: ,
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Product Specifications

Product SkuBCRECP-001474
Product DescriptionRecombinant Human Tyrosine-protein phosphatase non-receptor type 1 (PTPN1) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-321aa. [Accession | P18031].
Uniprot No.P18031
Gene NamesPTPN1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-321aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight44.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades.

Biological Context

Subcellular Location: Endoplasmic reticulum membrane (Peripheral membrane protein)
Tissue Specificity: Expressed in keratinocytes (at protein level)

Product Specifications

Recombinant Human Tyrosine-protein phosphatase non-receptor type 1 (PTPN1), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-321aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 44.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET

Subcellular Location

Endoplasmic reticulum membrane (Peripheral membrane protein)

Tissue Specificity

Expressed in keratinocytes (at protein level)

Subunit

Interacts with EPHA3 (phosphorylated); dephosphorylates EPHA3 and may regulate its trafficking and function (PubMed:21135139). Interacts with MET (PubMed:18819921). Interacts with NCK1 (PubMed:21707536)

Gene: PTPN1  |  Organism: Homo sapiens  |  Synonyms: Protein-tyrosine phosphatase 1B
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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