Protein Expression Service
High-quality proteins, antibodies and custom solutions — accelerating drug discovery, diagnostics and basic research.
With 12,000+ recombinant proteins delivered and a 90% project success rate, BioCrest Sci provides expert production of challenging targets — transmembrane proteins, kinases, enzymes, and viral antigens. Our proteins support drug discovery, antibody development, ELISA, structural biology, enzyme assays, and cell-based studies.
Why BioCrest Sci
One‑stop solution
Gene synthesis → cloning → expression → purification12,000+ proteins capacity
Viral antigens, cytokines, drug targets, enzymesμg to kg scale
Multiple expression systems (bacterial, yeast, insect, mammalian, cell‑free)Strict QC
Batch‑to‑batch consistency & activity validationProtein Expression Systems
Bacterial Protein Expression Service
✔ Fastest (3–5 weeks)
✔ Low cost, high yield
⚠ No glycosylation, possible solubility issues
✔ Fastest (3–5 weeks)
✔ Low cost, high yield
⚠ No glycosylation, possible solubility issues
Insect Baculovirus Expression Service
✔ Eukaryotic folding, moderate glycosylation
✔ Gram‑scale production
⚠ Longer optimization for secreted proteins
✔ Eukaryotic folding, moderate glycosylation
✔ Gram‑scale production
⚠ Longer optimization for secreted proteins
Yeast Protein Expression Service
✔ Scalable, high safety
✔ Suitable for multimeric & some membrane proteins
⚠ Glycosylation differs from mammals
✔ Scalable, high safety
✔ Suitable for multimeric & some membrane proteins
⚠ Glycosylation differs from mammals
Mammalian Protein Expression Service
✔ Native conformation, full glycosylation
✔ Secreted expression, easy purification
⚠ Higher cost, longer timeline
✔ Native conformation, full glycosylation
✔ Secreted expression, easy purification
⚠ Higher cost, longer timeline
Transmembrane Protein Expression Service
✔ Full-length membrane proteins (GPCRs, ion channels, transporters)
✔ Multiple display platforms available
✔ Native or near-native conformation
✔ Full-length membrane proteins (GPCRs, ion channels, transporters)
✔ Multiple display platforms available
✔ Native or near-native conformation
Cell-Free Protein Expression Service
✔ Rapid protein synthesis without live cells
✔ Ideal for toxic or membrane proteins
✔ High-throughput screening compatible
✔ Rapid protein synthesis without live cells
✔ Ideal for toxic or membrane proteins
✔ High-throughput screening compatible
Transmembrane Protein Display Platforms
VLP Platform
Displayed Transmembrane Proteins
Virus-like particle platform for membrane protein presentation in native-like environment
Detergent Platform
Displayed Transmembrane Proteins
Detergent-solubilized membrane proteins for biochemical and structural studies
Synthetic Nanodisc Platform
Displayed Transmembrane Proteins
Synthetic nanodisc-embedded membrane proteins for maximal stability and functionality
Expression System Selection Guide
| Primary Priority | Recombinant System | Turnaround Time | Relative Cost | Key Limitation | Main Applications |
|---|---|---|---|---|---|
| Fastest protein acquisition | Bacterial (E. coli) | 3–5 weeks | Low | No glycosylation; solubility | Antigens, cytosolic proteins, enzymes |
| Native conformation & glycosylation | Mammalian | 6–8 weeks | High | Lower expression yield | Therapeutic targets, functional studies, antibodies |
| Membrane proteins / VLPs | Insect (Baculovirus) | 6–8 weeks | Medium | Scale‑up complexity | Vaccine development, structural studies |
| Full-length transmembrane proteins | VLP / Detergent / Nanodisc / Cell-Free | 4–8 weeks | Medium-High | Platform selection critical | GPCRs, ion channels, transporters, functional analysis |
| Toxic or difficult proteins | Cell-Free | 2–4 weeks | Medium | Limited for large-scale | Toxic proteins, membrane proteins, rapid screening |
Service Process
Frequently Asked Questions
1. How do I choose the right expression system?
We recommend E. coli for speed & low cost (antigens, simple proteins). Mammalian for complex, glycosylated or membrane proteins. Insect for VLPs or when moderate glycosylation is acceptable. Cell-Free for toxic or difficult-to-express proteins. Contact us for a free recommendation based on your sequence.
2. What transmembrane protein platforms do you offer?
We offer three specialized platforms: VLP platform (virus-like particles), Detergent platform (detergent-solubilized), and Synthetic Nanodisc platform. For full-length transmembrane proteins, we also provide Cell-Free expression as a rapid option. Each is optimized for different downstream applications including immunization, drug screening, and structural biology.
3. What is the estimated cost & turnaround?
Cost depends on system, yield, purity requirements. Typical ranges: Bacterial from $800, Mammalian from $2,500+, Cell-Free from $1,500+. Turnaround: 2–8 weeks. We provide a fixed quote upfront.
4. Can you express full-length GPCRs or ion channels?
Yes. We specialize in full‑length transmembrane proteins including GPCRs, ion channels, and transporters using our VLP, nanodisc, detergent micelle platforms, or cell‑free systems.
5. Do you provide activity data?
Absolutely. Every protein batch includes QC data (SDS‑PAGE, endotoxin, concentration) and optional functional assays (enzyme activity, binding ELISA, ligand binding).
Featured Success
Full-length GPCR
Mammalian system + Nanodisc platform → high-purity, ligand binding confirmed.
Mammalian system + Nanodisc platform → high-purity, ligand binding confirmed.
SARS-CoV-2 RBD
Insect system → 50+ mg/L, used in ELISA kits worldwide.
Insect system → 50+ mg/L, used in ELISA kits worldwide.
Toxic Enzyme
Cell-free system → rapid expression, active conformation achieved.
Cell-free system → rapid expression, active conformation achieved.
Ion Channel (VLP platform)
Mammalian expression + VLP display → functional for antibody screening.
Mammalian expression + VLP display → functional for antibody screening.
Request a Quote / Contact Tech Team
Email: protein@biocrest.com | Fast response within 24h
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