Recombinant Human Large neutral amino acids transporter small subunit 1 (SLC7A5), partial, Homo sapiens (Human), E.coli, N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged, 19.7kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Large neutral amino acids transporter small subunit 1 (SLC7A5), partial

Recombinant Human Large neutral amino acids transporter small subunit 1 (SLC7A5), partial — recombinant protein from Homo sapiens (Human), expressed in E.coli. Purity >90%.

SKU: BCRECP-001658 Category:
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Product Specifications

Product SkuBCRECP-001658
Product DescriptionRecombinant Human Large neutral amino acids transporter small subunit 1 (SLC7A5) Protein is expressed from E.coli with N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged. It contains 16-50aa. [Accession | Q01650].
Uniprot No.Q01650
Gene NamesSLC7A5
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region16-50aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Molecular weight19.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

(Microbial infection) In case of hepatitis C virus/HCV infection, the complex formed by SLC3A2 and SLC7A5/LAT1 plays a role in HCV propagation by facilitating viral entry into host cell and increasing L-leucine uptake-mediated mTORC1 signaling activation, thereby contributing to HCV-mediated pathogenesis.

Biological Context

Subcellular Location: Apical cell membrane (Multi-pass membrane protein); Cell membrane (Multi-pass membrane protein); Lysosome membrane (Multi-pass membrane protein)
Tissue Specificity: Detected in placenta, in the syncytiotrophoblast layer (at protein level). Expressed abundantly in adult lung, liver, brain, skeletal muscle, placenta, bone marrow, testis, resting lymphocytes and monocytes, and in fetal liver. Weaker expression in thymus, cornea, retina, peripheral leukocytes, spleen, kidney, colon and lymph node. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Also expressed in all human tumor cell lines tested and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco-2

Product Specifications

Recombinant Human Large neutral amino acids transporter small subunit 1 (SLC7A5), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 16-50aa, with N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged tag, molecular weight 19.7kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

(Microbial infection) In case of hepatitis C virus/HCV infection, the complex formed by SLC3A2 and SLC7A5/LAT1 plays a role in HCV propagation by facilitating viral entry into host cell and increasing L-leucine uptake-mediated mTORC1 signaling activation, thereby contributing to HCV-mediated pathogenesis

Subcellular Location

Apical cell membrane (Multi-pass membrane protein); Cell membrane (Multi-pass membrane protein); Lysosome membrane (Multi-pass membrane protein)

Tissue Specificity

Detected in placenta, in the syncytiotrophoblast layer (at protein level) (PubMed:11389679). Expressed abundantly in adult lung, liver, brain, skeletal muscle, placenta, bone marrow, testis, resting lymphocytes and monocytes, and in fetal liver. Weaker expression in thymus, cornea, retina, peripheral leukocytes, spleen, kidney, colon and lymph node. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Also expressed in all human tumor cell lines tested and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco-2

Subunit

Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc (PubMed:10049700, PubMed:10574970, PubMed:11389679, PubMed:11557028, PubMed:11564694, PubMed:12117417, PubMed:12225859, PubMed:15769744, PubMed:25998567, PubMed:30867591, PubMed:9751058). Interacts with LAPTM4B; this recruits the heterodimer formed by SLC3A2/4F2hc and SLC7A5 to lysosomes to promote leucine uptake into these organelles and is required for mTORC1 activation (PubMed:25998567)

Gene: SLC7A5  |  Organism: Homo sapiens  |  Synonyms: 4F2 light chain; CD98 light chain; Integral membrane protein E16; L-type amino acid transporter 1; Solute carrier family 7 member 5; y+ system cationic amino acid transporter
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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