Recombinant Human E3 ubiquitin-protein ligase ZFP91 (ZFP91), partial, Homo sapiens (Human), E.coli, N-terminal 6xHis-B2M-tagged, 51.5kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human E3 ubiquitin-protein ligase ZFP91 (ZFP91), partial

Recombinant Human E3 ubiquitin-protein ligase ZFP91 (ZFP91), partial — DNA-binding transcription factor. Purity >90%.

SKU: BCRECP-001935 Categories: ,
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Product Specifications

Product SkuBCRECP-001935
Product DescriptionRecombinant Human E3 ubiquitin-protein ligase ZFP91 (ZFP91) Protein is expressed from E.coli with N-terminal 6xHis-B2M-tagged. It contains 242-570aa. [Accession | Q96JP5].
Uniprot No.Q96JP5
Gene NamesZFP91
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region242-570aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-B2M-tagged
Molecular weight51.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasEpigenetics and Nuclear Signaling

Function

DNA-binding transcription factor. Also acts as an atypical E3 ubiquitin-protein ligase that mediates 'Lys-63'-linked ubiquitination of MAP3K14/NIK, leading to stabilize and activate MAP3K14/NIK. It thereby acts as an activator of the non-canonical NF-kappa-B2/NFKB2 pathway.

Biological Context

Subcellular Location: Nucleus
Tissue Specificity: Expressed ubiquitously, particularly at high level in testis. Isoform 2 is testis specific
Pathway: Protein modification; protein ubiquitination

Product Specifications

Recombinant Human E3 ubiquitin-protein ligase ZFP91 (ZFP91), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 242-570aa, with N-terminal 6xHis-B2M-tagged tag, molecular weight 51.5kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

DNA-binding transcription factor (PubMed:34936696, PubMed:40608935). Also acts as an atypical E3 ubiquitin-protein ligase that mediates 'Lys-63'-linked ubiquitination of MAP3K14/NIK, leading to stabilize and activate MAP3K14/NIK (PubMed:20682767). It thereby acts as an activator of the non-canonical NF-kappa-B2/NFKB2 pathway (PubMed:20682767). May also play an important role in cell proliferation and/or anti-apoptosis (PubMed:12738986)

Subcellular Location

Nucleus

Tissue Specificity

Expressed ubiquitously, particularly at high level in testis. Isoform 2 is testis specific

Subunit

Interacts with Zincore complex components QRICH1 and SEPHS1; stabilizing ZFP91 at DNA-binding sites across the genome

Pathway

Protein modification; protein ubiquitination

Gene: ZFP91  |  Organism: Homo sapiens  |  Synonyms: RING-type E3 ubiquitin transferase ZFP91; Zinc finger protein 757; Zinc finger protein 91 homolog
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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