Recombinant Mouse Myosin regulatory light chain 2, ventricular/cardiac muscle isoform (Myl2), Mus musculus (Mouse), E.coli, C-terminal 6xHis-tagged, 25.6kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mouse Myosin regulatory light chain 2, ventricular/cardiac muscle isoform (Myl2)

Recombinant Mouse Myosin regulatory light chain 2, ventricular/cardiac muscle isoform (Myl2) — Contractile protein that plays a role in heart development and function. Purity >90%.

SKU: BCRECP-003446 Category:
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Product Specifications

Product SkuBCRECP-003446
Product DescriptionRecombinant Mouse Myosin regulatory light chain 2, ventricular/cardiac muscle isoform (Myl2) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 2-166aa. [Accession | P51667].
Uniprot No.P51667
Gene NamesMyl2
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-166aa
SpeciesMus musculus (Mouse)
Tag InfoC-terminal 6xHis-tagged
Molecular weight25.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Contractile protein that plays a role in heart development and function. Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force.

Product Specifications

Recombinant Mouse Myosin regulatory light chain 2, ventricular/cardiac muscle isoform (Myl2) — Contractile protein that plays a role in heart development and function. Purity >90%. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Contractile protein that plays a role in heart development and function (PubMed:10409661). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (By similarity) (PubMed:10409661, PubMed:16908724, PubMed:22426213). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (PubMed:9422794)

Subcellular Location

Cytoplasm, myofibril, sarcomere, A band

Tissue Specificity

Abundantly expressed in both cardiac and slow skeletal muscle (PubMed:1379240). In the adult heart, the phosphorylated form is highly expressed in epicardium and weakly in endocardium (PubMed:22426213)

Subunit

Myosin is a hexamer of 2 heavy chains and 4 light chains (By similarity). Interacts with MYOC (By similarity)

Gene: Myl2  |  Organism: Mus musculus  |  Synonyms: Myosin light chain 2, slow skeletal/ventricular muscle isoform
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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