Recombinant Severe acute respiratory syndrome coronavirus Nucleoprotein (N), Severe acute respiratory syndrome coronavirus (SARS-CoV), E.coli, N-terminal 6xHis-tagged, 50.1kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Severe acute respiratory syndrome coronavirus Nucleoprotein (N)

Recombinant Severe acute respiratory syndrome coronavirus Nucleoprotein (N) — Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane p… Purity >85%.

SKU: BCRECP-003484 Categories: , , ,
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Product Specifications

Product SkuBCRECP-003484
Product DescriptionRecombinant Severe acute respiratory syndrome coronavirus Nucleoprotein (N) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-422aa. [Accession | P59595].
Uniprot No.P59595
Gene NamesN
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-422aa
SpeciesSevere acute respiratory syndrome coronavirus (SARS-CoV)
Tag InfoN-terminal 6xHis-tagged
Molecular weight50.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.

Product Specifications

Recombinant Severe acute respiratory syndrome coronavirus Nucleoprotein (N) — Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane p… Purity >85%. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication (PubMed:17210170). May modulate transforming growth factor-beta signaling by binding host SMAD3 (PubMed:18055455)

Subcellular Location

Virion; Host endoplasmic reticulum-Golgi intermediate compartment; Host Golgi apparatus; Host cytoplasm, host perinuclear region; Host nucleus

Subunit

Homooligomer. Both monomeric and oligomeric forms interact with RNA. Interacts with protein M (PubMed:15351485). Interacts with protein E (PubMed:24766657). May bind to host HNRNPA1 (Probable). Interacts with NSP3; this interaction serves to tether the genome to the newly translated replicase-transcriptase complex at a very early stage of infection (By similarity). May interact with host SMAD3 (Probable). Interacts with host PPIA/CYPA (PubMed:15688292)

Gene: N  |  Organism: Severe acute respiratory syndrome coronavirus  |  Synonyms: Nucleocapsid protein
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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