Recombinant Androctonus mauretanicus mauretanicus Alpha-toxin Amm8, Androctonus mauritanicus mauritanicus (Scorpion), Yeast, N-terminal 6xHis-tagged, 9.4kDa, purity >90% as determined by SDS-PAGE.

Recombinant Androctonus mauretanicus mauretanicus Alpha-toxin Amm8

Recombinant Androctonus mauretanicus mauretanicus Alpha-toxin Amm8 — Alpha toxins bind voltage-independently at site-3 of sodium channels (Nav) and inhibit the inactivation of the activated channels, thereby blocking neuronal transmission. Purity >90%.

SKU: BCRECP-003833 Category:
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Product Specifications

Product SkuBCRECP-003833
Product DescriptionRecombinant Androctonus mauretanicus mauretanicus Alpha-toxin Amm8 Protein is expressed from Yeast with N-terminal 6xHis-tagged. It contains 20-84aa. [Accession | Q7YXD3].
Uniprot No.Q7YXD3
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region20-84aa
SpeciesAndroctonus mauritanicus mauritanicus (Scorpion)
Tag InfoN-terminal 6xHis-tagged
Molecular weight9.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Alpha toxins bind voltage-independently at site-3 of sodium channels (Nav) and inhibit the inactivation of the activated channels, thereby blocking neuronal transmission. The toxin principally slows the inactivation process of TTX-sensitive sodium channels. It discriminates neuronal versus muscular sodium channel, as it is more potent on rat brain Nav1.2/SCN2A (EC(50)=29 nM) than on rat skeletal muscle Nav1.4/SCN4A (EC(50)=416 nM).

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Expressed by the venom gland

Product Specifications

Recombinant Androctonus mauretanicus mauretanicus Alpha-toxin Amm8 is a recombinant protein from Androctonus mauritanicus mauritanicus (Scorpion), expressed in Yeast, covering amino acids 20-84aa, with N-terminal 6xHis-tagged tag, molecular weight 9.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Alpha toxins bind voltage-independently at site-3 of sodium channels (Nav) and inhibit the inactivation of the activated channels, thereby blocking neuronal transmission (PubMed:12911331). The toxin principally slows the inactivation process of TTX-sensitive sodium channels (PubMed:23685008). It discriminates neuronal versus muscular sodium channel, as it is more potent on rat brain Nav1.2/SCN2A (EC(50)=29 nM) than on rat skeletal muscle Nav1.4/SCN4A (EC(50)=416 nM) (PubMed:12911331). It also shows a weak activity on Nav1.7/SCN9A (EC(50)=1.76 uM) (PubMed:23685008). In vivo, the toxin produces pain hypersensibility to mechanical and thermal stimuli (PubMed:23685008). It also exhibits potent analgesic activity (when injected intraperitoneally), increasing hot plate and tail flick withdrawal latencies in a dose-dependent fashion (PubMed:20619318). This paradoxical analgesic action, is significantly suppressed by opioid receptor antagonists, suggesting a pain-induced analgesia mechanism that involves an endogenous opioid system (PubMed:20619318). This led to hypothesis that pain relief induced by peripheral administration of Amm VIII may result from sensitization of primary afferent neurons and subsequent activation of an opioid-dependent noxious inhibitory control (PubMed:20619318)

Subcellular Location

Secreted

Tissue Specificity

Expressed by the venom gland

Organism: Androctonus mauritanicus mauritanicus  |  Synonyms: Alpha-anatoxin Amm VIII; Neurotoxin 8; P4

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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