Recombinant Influenza A virus Nucleoprotein (NP), Influenza A virus (strain A/Port Chalmers/1/1973 H3N2), E.coli, N-terminal 6xHis-SUMO-tagged, 72.1kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Influenza A virus Nucleoprotein (NP)

Recombinant Influenza A virus Nucleoprotein (NP) — Encapsidates the negative strand viral RNA, protecting it from nucleases. Purity >90%.

SKU: BCRECP-003895 Categories: , ,
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Product Specifications

Product SkuBCRECP-003895
Product DescriptionRecombinant Influenza A virus Nucleoprotein (NP) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 1-498aa. [Accession | Q1PUD5].
Uniprot No.Q1PUD5
Gene NamesNP
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-498aa
SpeciesInfluenza A virus (strain A/Port Chalmers/1/1973 H3N2)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight72.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex.

Biological Context

Subcellular Location: Virion; Host nucleus

Product Specifications

Recombinant Influenza A virus Nucleoprotein (NP) is a recombinant protein from Influenza A virus (strain A/Port Chalmers/1/1973 H3N2), expressed in E.coli, covering amino acids 1-498aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 72.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus

Subcellular Location

Virion; Host nucleus

Subunit

Homomultimerizes to form the nucleocapsid. May bind host exportin-1/XPO1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases

Gene: NP  |  Organism: Influenza A virus (strain A/Port Chalmers/1/1973 H3N2)  |  Synonyms: Nucleocapsid protein

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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