Recombinant Escherichia coli Hydrogen peroxide-inducible genes activator (oxyR), Biotinylated, Escherichia coli (strain K12), E.coli, N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged, 82.1kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Escherichia coli Hydrogen peroxide-inducible genes activator (oxyR), Biotinylated

Recombinant Escherichia coli Hydrogen peroxide-inducible genes activator (oxyR), Biotinylated — Hydrogen peroxide (H2O2) sensor. Purity >90%.

SKU: BCRECP-003987 Categories: ,
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Product Specifications

Product SkuBCRECP-003987
Product DescriptionRecombinant Escherichia coli Hydrogen peroxide-inducible genes activator (oxyR) Protein is expressed from E.coli with N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged. It contains 1-305aa. [Accession | P0ACQ4].
Uniprot No.P0ACQ4
Gene NamesoxyR
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-305aa
SpeciesEscherichia coli (strain K12)
Tag InfoN-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged
Molecular weight82.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Hydrogen peroxide (H2O2) sensor. Activates the expression of a regulon of H2O2-inducible genes such as katG, gor, ahpC, ahpF, oxyS (a regulatory RNA), dps, fur and grxA in response to H2O2. Represses transcription of phage Mu mom gene in a methylation-sensitive manner; MomR binds to the mom promoter when it is unmethylated but not if it is fully methylated.

Product Specifications

Recombinant Escherichia coli Hydrogen peroxide-inducible genes activator (oxyR), Biotinylated is a recombinant protein from Escherichia coli (strain K12), expressed in E.coli, covering amino acids 1-305aa, with N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged tag, molecular weight 82.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Hydrogen peroxide (H2O2) sensor. Activates the expression of a regulon of H2O2-inducible genes such as katG, gor, ahpC, ahpF, oxyS (a regulatory RNA), dps, fur and grxA in response to H2O2. Represses transcription of phage Mu mom gene in a methylation-sensitive manner; MomR binds to the mom promoter when it is unmethylated but not if it is fully methylated (PubMed:2551682). Binds DNA in the upstream regions of its target genes; more than one site may be present per gene (PubMed:1864839, PubMed:2471187, PubMed:2551682). OxyR is inactivated by reduction of its essential disulfide bond by glutaredoxin (Grx1, grxA), itself positively regulated by OxyR (PubMed:9497290). Also has a positive regulatory effect on the production of surface proteins that control the colony morphology and auto-aggregation ability (PubMed:15659678)

Subunit

Homodimer and homotetramer

Gene: oxyR  |  Organism: Escherichia coli (strain K12)  |  Synonyms: Hydrogen peroxide-inducible genes activator; Morphology and auto-aggregation control protein
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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