Recombinant Agrobacterium fabrum Single-strand DNA-binding protein (virE2), Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58)), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 70.3kDa, purity >85% as determined by SDS-PAGE.

Recombinant Agrobacterium fabrum Single-strand DNA-binding protein (virE2)

Recombinant Agrobacterium fabrum Single-strand DNA-binding protein (virE2) — Involved in DNA transformation; mediates the nuclear uptake of single-stranded DNA copies of the transferred DNA (T-DNA) element. Purity >85%.

SKU: BCRECP-004728 Category:
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Product Specifications

Product SkuBCRECP-004728
Product DescriptionRecombinant Agrobacterium fabrum Single-strand DNA-binding protein (virE2) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-556aa. [Accession | P08062].
Uniprot No.P08062
Gene NamesVIRE2
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-556aa
SpeciesAgrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight70.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Involved in DNA transformation; mediates the nuclear uptake of single-stranded DNA copies of the transferred DNA (T-DNA) element. Binds single-stranded but not double-stranded DNA regardless of nucleotide sequence composition.

Biological Context

Subcellular Location: Secreted; Host nucleus

Product Specifications

Recombinant Agrobacterium fabrum Single-strand DNA-binding protein (virE2) is a recombinant protein from Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58)), expressed in E.coli, covering amino acids 1-556aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 70.3kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Involved in DNA transformation; mediates the nuclear uptake of single-stranded DNA copies of the transferred DNA (T-DNA) element. Binds single-stranded but not double-stranded DNA regardless of nucleotide sequence composition

Subcellular Location

Secreted; Host nucleus

Subunit

Interacts with host VIP2 that promotes T-DNA integration into the host genome. Forms a complex made of virE2 and host proteins VIP1 and VBF (By similarity). Forms heterodimers with the chaperone protein virE1 that prevent virE2 anarchic homopolymerization. Interacts with A.thaliana VIP1 that mediates its translocation to the host nucleus. Forms a complex made of VirE2, host VIP1 and VIP2 and single-stranded DNA (ssDNA)

Gene: virE2  |  Organism: Agrobacterium fabrum (strain C58 / ATCC 33970)  |  Synonyms: 63.5 kDa virulence protein
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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