Recombinant African swine fever virus Inner membrane protein p54 (Ba71V-126), partial, African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV), E.coli, N-terminal 6xHis-tagged, 17.8kDa, purity >90% as determined by SDS-PAGE.
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Recombinant African swine fever virus Inner membrane protein p54 (Ba71V-126), partial

Recombinant African swine fever virus Inner membrane protein p54 (Ba71V-126), partial — Inner envelope protein involved, through its interaction with host dynein, in the intracellular microtubule-dependent transport of viral capsid toward viral factories. Purity >90%.

SKU: BCRECP-002172 Categories: ,
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Product Specifications

Product SkuBCRECP-002172
Product DescriptionRecombinant African swine fever virus Inner membrane protein p54 (Ba71V-126) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 54-183aa. [Accession | Q65194].
Uniprot No.Q65194
Gene NamesBa71V-126
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region54-183aa
SpeciesAfrican swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)
Tag InfoN-terminal 6xHis-tagged
Molecular weight17.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Inner envelope protein involved, through its interaction with host dynein, in the intracellular microtubule-dependent transport of viral capsid toward viral factories. Seems to induce caspase-3 activation and apoptosis. Plays a role in virion morphogenesis by recruiting and transforming the host ER membranes into the precursors of the viral envelope.

Biological Context

Subcellular Location: Virion membrane (Single-pass membrane protein); Host cytoplasm, host cytoskeleton; Host endoplasmic reticulum membrane

Product Specifications

Recombinant African swine fever virus Inner membrane protein p54 (Ba71V-126), partial is a recombinant protein from African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV), expressed in E.coli, covering amino acids 54-183aa, with N-terminal 6xHis-tagged tag, molecular weight 17.8kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Inner envelope protein involved, through its interaction with host dynein, in the intracellular microtubule-dependent transport of viral capsid toward viral factories (PubMed:11559815). Seems to induce caspase-3 activation and apoptosis (PubMed:15225638). Plays a role in virion morphogenesis by recruiting and transforming the host ER membranes into the precursors of the viral envelope (PubMed:15047843). Involved in virus attachment to the host cell (PubMed:8764090, PubMed:9568043)

Subcellular Location

Virion membrane (Single-pass membrane protein); Host cytoplasm, host cytoskeleton; Host endoplasmic reticulum membrane

Subunit

Interacts with the host light chain cytoplasmic dynein DYNLL1; this interaction is critical for intracellular microtubule-dependent virus transport toward viral factories

Organism: African swine fever virus (strain Badajoz 1971 Vero-adapted)  |  Synonyms: pE183L
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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