Recombinant Clostridium perfringens Microbial collagenase (colA), partial, Clostridium perfringens (strain 13 / Type A), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 39.7kDa, purity >85% as determined by SDS-PAGE.

Recombinant Clostridium perfringens Microbial collagenase (colA), partial

Recombinant Clostridium perfringens Microbial collagenase (colA), partial — Clostridial collagenases are among the most efficient degraders of eukaryotic collagen known; saprophytes use collagen as a carbon source while pathogens additionally digest collagen to aid in host… Purity >85%.

SKU: BCRECP-002411 Category:
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Product Specifications

Product SkuBCRECP-002411
Product DescriptionRecombinant Clostridium perfringens Microbial collagenase (colA) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 90-370aa. [Accession | P43153].
Uniprot No.P43153
Gene NamescolA
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region90-370aa
SpeciesClostridium perfringens (strain 13 / Type A)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight39.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research Areasothers

Function

Clostridial collagenases are among the most efficient degraders of eukaryotic collagen known; saprophytes use collagen as a carbon source while pathogens additionally digest collagen to aid in host colonization. Has both tripeptidylcarboxypeptidase on Gly-X-Y and endopeptidase activities; the endopeptidase cuts within the triple helix region of collagen while tripeptidylcarboxypeptidase successively digests the exposed ends, thus clostridial collagenases can digest large sections of collagen.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Clostridium perfringens Microbial collagenase (colA), partial is a recombinant protein from Clostridium perfringens (strain 13 / Type A), expressed in E.coli, covering amino acids 90-370aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 39.7kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Clostridial collagenases are among the most efficient degraders of eukaryotic collagen known; saprophytes use collagen as a carbon source while pathogens additionally digest collagen to aid in host colonization. Has both tripeptidylcarboxypeptidase on Gly-X-Y and endopeptidase activities; the endopeptidase cuts within the triple helix region of collagen while tripeptidylcarboxypeptidase successively digests the exposed ends, thus clostridial collagenases can digest large sections of collagen (By similarity)

Subcellular Location

Secreted

Gene: colA  |  Organism: Clostridium perfringens (strain 13 / Type A)  |  Synonyms: 120 kDa collagenase; Microbial collagenase

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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