Recombinant African swine fever virus Inner membrane protein p54 (p54), partial, African swine fever virus (ASFV), E.coli, C-terminal 6xHis-tagged, 20.9kDa, purity >95% as determined by SDS-PAGE.
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Recombinant African swine fever virus Inner membrane protein p54 (p54), partial

Recombinant African swine fever virus Inner membrane protein p54 (p54), partial — Inner envelope protein involved, through its interaction with host dynein, in the intracellular microtubule-dependent transport of viral capsid toward viral factories. Purity >95%.

SKU: BCRECP-004001 Categories: ,
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Product Specifications

Product SkuBCRECP-004001
Product DescriptionRecombinant African swine fever virus Inner membrane protein p54 (p54) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 53-184aa. [Accession | B2MUM6].
Uniprot No.B2MUM6
Gene Namesp54
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region53-184aa
SpeciesAfrican swine fever virus (ASFV)
Tag InfoC-terminal 6xHis-tagged
Molecular weight20.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Inner envelope protein involved, through its interaction with host dynein, in the intracellular microtubule-dependent transport of viral capsid toward viral factories. Seems to induce caspase-3 activation and apoptosis. Plays a role in virion morphogenesis by recruiting and transforming the host ER membranes into the precursors of the viral envelope.

Biological Context

Subcellular Location: Host cytoplasm, host cytoskeleton; Host endoplasmic reticulum membrane; Virion membrane (Single-pass membrane protein)

Product Specifications

Recombinant African swine fever virus Inner membrane protein p54 (p54), partial is a recombinant protein from African swine fever virus (ASFV), expressed in E.coli, covering amino acids 53-184aa, with C-terminal 6xHis-tagged tag, molecular weight 20.9kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Inner envelope protein involved, through its interaction with host dynein, in the intracellular microtubule-dependent transport of viral capsid toward viral factories. Seems to induce caspase-3 activation and apoptosis. Plays a role in virion morphogenesis by recruiting and transforming the host ER membranes into the precursors of the viral envelope. Involved in virus attachment to the host cell

Subcellular Location

Host cytoplasm, host cytoskeleton; Host endoplasmic reticulum membrane; Virion membrane (Single-pass membrane protein)

Subunit

Interacts with the host light chain cytoplasmic dynein DYNLL1; this interaction is critical for intracellular microtubule-dependent virus transport toward viral factories

Gene: p54  |  Organism: African swine fever virus  |  Synonyms: pE183L
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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