Recombinant Apis mellifera Hyaluronidase, Apis mellifera (Honeybee), Yeast, C-terminal 6xHis-tagged, 42.2kDa, purity >95% as determined by SDS-PAGE.

Recombinant Apis mellifera Hyaluronidase

Recombinant Apis mellifera Hyaluronidase — recombinant protein from Apis mellifera (Honeybee), expressed in Yeast. Purity >95%.

SKU: BCRECP-003823 Category:
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Product Specifications

Product SkuBCRECP-003823
Product DescriptionRecombinant Apis mellifera Hyaluronidase Protein is expressed from Yeast with C-terminal 6xHis-tagged. It contains 34-382aa. [Accession | Q08169].
Uniprot No.Q08169
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region34-382aa
SpeciesApis mellifera (Honeybee)
Tag InfoC-terminal 6xHis-tagged
Molecular weight42.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Venom endo-hyaluronidase that acts as a spreading factor by hydrolyzing hyaluronic acid (HA) in the extracellular matrix, aiding diffusion of other venom components.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Expressed in the venom glands of worker bees. Is also detected in the testes of drones but not in the queen-bee venom glands or in pupae (at transcript level)

Product Specifications

Recombinant Apis mellifera Hyaluronidase is a recombinant protein from Apis mellifera (Honeybee), expressed in Yeast, covering amino acids 34-382aa, with C-terminal 6xHis-tagged tag, molecular weight 42.2kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Venom endo-hyaluronidase that acts as a spreading factor by hydrolyzing hyaluronic acid (HA) in the extracellular matrix, aiding diffusion of other venom components

Subcellular Location

Secreted

Tissue Specificity

Expressed in the venom glands of worker bees. Is also detected in the testes of drones but not in the queen-bee venom glands or in pupae (at transcript level)

Organism: Apis mellifera  |  Synonyms: Allergen Api m II; Hyaluronoglucosaminidase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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