Recombinant Bovine NADPH:adrenodoxin oxidoreductase, mitochondrial (FDXR), Bos taurus (Bovine), Yeast, N-terminal 10xHis-tagged, 52.8kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Bovine NADPH:adrenodoxin oxidoreductase, mitochondrial (FDXR)

Recombinant Bovine NADPH:adrenodoxin oxidoreductase, mitochondrial (FDXR) — Serves as the first electron transfer protein in all the mitochondrial P450 systems including cholesterol side chain cleavage in all steroidogenic tissues, steroid 11-beta hydroxylation in the adre… Purity >85%.

SKU: BCRECP-002719 Categories: ,
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Product Specifications

Product SkuBCRECP-002719
Product DescriptionRecombinant Bovine NADPH:adrenodoxin oxidoreductase, mitochondrial (FDXR) Protein is expressed from Yeast with N-terminal 10xHis-tagged. It contains 33-492aa. [Accession | P08165].
Uniprot No.P08165
Gene NamesFDXR
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region33-492aa
SpeciesBos taurus (Bovine)
Tag InfoN-terminal 10xHis-tagged
Molecular weight52.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCell Biology

Function

Serves as the first electron transfer protein in all the mitochondrial P450 systems including cholesterol side chain cleavage in all steroidogenic tissues, steroid 11-beta hydroxylation in the adrenal cortex, 25-OH-vitamin D3-24 hydroxylation in the kidney, and sterol C-27 hydroxylation in the liver. Also acts as a ferredoxin–NADP(+) reductase essential for coenzyme Q biosynthesis: together with FDX2, transfers the electrons required for the hydroxylation reaction performed by COQ6.

Biological Context

Subcellular Location: Mitochondrion inner membrane (Peripheral membrane protein)
Tissue Specificity: Detected in adrenal cortex and corpus luteum (at protein level)
Pathway: Steroid metabolism; cholesterol metabolism

Product Specifications

Recombinant Bovine NADPH:adrenodoxin oxidoreductase, mitochondrial (FDXR) is a recombinant protein from Bos taurus (Bovine), expressed in Yeast, covering amino acids 33-492aa, with N-terminal 10xHis-tagged tag, molecular weight 52.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Serves as the first electron transfer protein in all the mitochondrial P450 systems including cholesterol side chain cleavage in all steroidogenic tissues, steroid 11-beta hydroxylation in the adrenal cortex, 25-OH-vitamin D3-24 hydroxylation in the kidney, and sterol C-27 hydroxylation in the liver (PubMed:7811729). Also acts as a ferredoxin--NADP(+) reductase essential for coenzyme Q biosynthesis: together with FDX2, transfers the electrons required for the hydroxylation reaction performed by COQ6 (By similarity)

Subcellular Location

Mitochondrion inner membrane (Peripheral membrane protein)

Tissue Specificity

Detected in adrenal cortex and corpus luteum (at protein level)

Subunit

Monomer. Interacts directly with FDX1

Pathway

Steroid metabolism; cholesterol metabolism

Gene: FDXR  |  Organism: Bos taurus  |  Synonyms: Ferredoxin--NADP(+) reductase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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