Recombinant Collariella virescens LPMO lytic polysaccharide monooxygenase (aa9), Collariella virescens, Yeast, C-terminal 6xHis-tagged, 29.2kDa, purity >90% as determined by SDS-PAGE.

Recombinant Collariella virescens LPMO lytic polysaccharide monooxygenase (aa9)

Recombinant Collariella virescens LPMO lytic polysaccharide monooxygenase (aa9) — Lytic polysaccharide monooxygenase (LPMO) that depolymerizes crystalline and amorphous polysaccharides via the oxidation of scissile alpha- or beta-(1-4)-glycosidic bonds, yielding C4 oxidation pro… Purity >90%.

SKU: BCRECP-001992 Category:
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Product Specifications

Product SkuBCRECP-001992
Product DescriptionRecombinant Collariella virescens LPMO lytic polysaccharide monooxygenase (aa9) Protein is expressed from Yeast with C-terminal 6xHis-tagged. It contains 23-274aa. [Accession | A0A223GEC9].
Uniprot No.A0A223GEC9
Gene Namesaa9
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region23-274aa
SpeciesCollariella virescens
Tag InfoC-terminal 6xHis-tagged
Molecular weight29.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Lytic polysaccharide monooxygenase (LPMO) that depolymerizes crystalline and amorphous polysaccharides via the oxidation of scissile alpha- or beta-(1-4)-glycosidic bonds, yielding C4 oxidation products. Catalysis by LPMOs requires the reduction of the active-site copper from Cu(II) to Cu(I) by a reducing agent and H(2)O(2) or O(2) as a cosubstrate. Cleaves a range of polysaccharides, including cellulose, xyloglucan, mixed-linkage glucan and glucomannan.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Collariella virescens LPMO lytic polysaccharide monooxygenase (aa9) is a recombinant protein from Collariella virescens, expressed in Yeast, covering amino acids 23-274aa, with C-terminal 6xHis-tagged tag, molecular weight 29.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Lytic polysaccharide monooxygenase (LPMO) that depolymerizes crystalline and amorphous polysaccharides via the oxidation of scissile alpha- or beta-(1-4)-glycosidic bonds, yielding C4 oxidation products (PubMed:29057953, PubMed:32818374). Catalysis by LPMOs requires the reduction of the active-site copper from Cu(II) to Cu(I) by a reducing agent and H(2)O(2) or O(2) as a cosubstrate (PubMed:29057953). Cleaves a range of polysaccharides, including cellulose, xyloglucan, mixed-linkage glucan and glucomannan (PubMed:29057953, PubMed:32818374)

Subcellular Location

Secreted

Gene: AA9A  |  Organism: Achaetomiella virescens  |  Synonyms: Cellulase AA9A; Endo-beta-1,4-glucanase AA9A; Glycosyl hydrolase 61 family protein AA9A

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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