Recombinant Epstein-Barr virus Tegument protein BGLF2 (BGLF2), Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 44.3kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Epstein-Barr virus Tegument protein BGLF2 (BGLF2)

Recombinant Epstein-Barr virus Tegument protein BGLF2 (BGLF2) — Plays a critical role in cytoplasmic virus egress. Purity >90%.

SKU: BCRECP-002194 Categories: ,
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Product Specifications

Product SkuBCRECP-002194
Product DescriptionRecombinant Epstein-Barr virus Tegument protein BGLF2 (BGLF2) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-336aa. [Accession | P0CK53].
Uniprot No.P0CK53
Gene NamesBGLF2
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-336aa
SpeciesEpstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight44.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Plays a critical role in cytoplasmic virus egress. Participates in the final step of tegumentation and envelope acquisition within the host cytoplasm by directly interacting with the capsid. Upon virion binding to target cell, a signaling cascade is triggered to disrupt the interaction with the capsid, thereby preparing capsid uncoating.

Biological Context

Subcellular Location: Virion tegument; Host cytoplasm; Host nucleus; Host Golgi apparatus, host trans-Golgi network

Product Specifications

Recombinant Epstein-Barr virus Tegument protein BGLF2 (BGLF2) is a recombinant protein from Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4), expressed in E.coli, covering amino acids 1-336aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 44.3kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Plays a critical role in cytoplasmic virus egress. Participates in the final step of tegumentation and envelope acquisition within the host cytoplasm by directly interacting with the capsid. Upon virion binding to target cell, a signaling cascade is triggered to disrupt the interaction with the capsid, thereby preparing capsid uncoating. Activates the AP-1 pathway and enhances EBV reactivation and virus release (PubMed:26559845, PubMed:29695622). Inhibits type I IFN-induced TYK2, STAT1 and STAT3 phosphorylation, thereby impairing type I IFN signaling and counteracting the ability of type I IFN to suppress the reactivation of EBV (PubMed:32213613, PubMed:34319780). Recruits SHP1 phosphatase to dephosphorylate STAT1 (PubMed:34319780). Mediates STAT2 ubiquitination and proteasomal degradation (PubMed:34319780). Also suppresses type II and type III IFN signaling (PubMed:34319780). Contributes to G1/S arrest in the host cell (PubMed:24501404). Acts as an miRNA regulator that interferes with the function of RISC in miRNA-mediated mRNA silencing (PubMed:35007297). As a result, SUMOylation is increased (PubMed:35007297). When encapsulated in the exosomes released by EBV-infected host cells, may facilitate the infection in recipient cells (By similarity) (PubMed:35729616)

Subcellular Location

Virion tegument; Host cytoplasm; Host nucleus; Host Golgi apparatus, host trans-Golgi network

Subunit

Homodimer (PubMed:26559845). Interacts with BBLF1 (PubMed:32038519). Interacts with the capsid. Interacts with BKRF4 (via C-terminus); this interaction is important for infectious virion production (PubMed:28904200). Interacts with host TYK2; this interaction participates to the inhibition of host type I IFN signaling (PubMed:32213613). Interacts with host STAT1; this interaction leads to STAT1 dephosphorylation and inhibition (PubMed:34319780). Interacts with host STAT2; this interaction leads to STAT2 degradation (PubMed:34319780). Interacts with host CUL1; this interaction might facilitate CUL1 recruitment to STAT2, leading to ubiquitination and degradation of the latter (PubMed:34319780). Interacts with host AGO2; this interaction participates to the regulation of the host miRNA leading to enhanced SUMOylation (By similarity) (PubMed:35007297)

Organism: Epstein-Barr virus (strain B95-8)  |  Synonyms: Tegument protein BGLF2
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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