Recombinant Escherichia coli Peptidyl-lysine N-acetyltransferase PatZ (patZ), partial, Escherichia coli (strain K12), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 26.3kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Escherichia coli Peptidyl-lysine N-acetyltransferase PatZ (patZ), partial

Recombinant Escherichia coli Peptidyl-lysine N-acetyltransferase PatZ (patZ), partial — Catalyzes the acetyl-CoA-dependent acetylation of lysine residues of a large number of target proteins. Purity >90%.

SKU: BCRECP-004027 Categories: ,
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Product Specifications

Product SkuBCRECP-004027
Product DescriptionRecombinant Escherichia coli Peptidyl-lysine N-acetyltransferase PatZ (patZ) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 724-886aa. [Accession | P76594].
Uniprot No.P76594
Gene NamespatZ
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region724-886aa
SpeciesEscherichia coli (strain K12)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight26.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Catalyzes the acetyl-CoA-dependent acetylation of lysine residues of a large number of target proteins. Acetylates RNase R in exponential phase cells and RNase II. Required for the glucose-dependent acetylation on multiple lysines of alpha, beta and beta' RNAP subunits.

Product Specifications

Recombinant Escherichia coli Peptidyl-lysine N-acetyltransferase PatZ (patZ), partial is a recombinant protein from Escherichia coli (strain K12), expressed in E.coli, covering amino acids 724-886aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 26.3kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Catalyzes the acetyl-CoA-dependent acetylation of lysine residues of a large number of target proteins (PubMed:21981926, PubMed:21696463, PubMed:22124017, PubMed:26847092, PubMed:30352934, PubMed:40498448). Acetylates RNase R in exponential phase cells and RNase II (PubMed:21981926, PubMed:22124017, PubMed:26847092). Required for the glucose-dependent acetylation on multiple lysines of alpha, beta and beta' RNAP subunits (PubMed:21696463). Also acetylates acetyl-coenzyme A synthetase (Acs) at 'Lys-609' and the chromosomal replication initiator protein DnaA, inhibiting their activity (PubMed:22059728, PubMed:26251518, PubMed:27484197, PubMed:40498448). But does not acetylate o-succinylbenzoyl-CoA synthetase (MenE) (PubMed:39422507). Overexpression leads to the acetylation of a large number of additional proteins and inhibits motility (PubMed:30352934)

Subunit

Homotetramer (PubMed:26251518, PubMed:40498448). Oligomerizes to an octameric form by autoacetylation (PubMed:26251518). Each unit contains a catalytic and a regulatory domain which binds two acetyl-CoA and one phosphate (PubMed:40498448)

Gene: patZ  |  Organism: Escherichia coli (strain K12)  |  Synonyms: Protein lysine acetyltransferase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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