Recombinant Human Aldo-keto reductase family 1 member B10 (AKR1B10), Homo sapiens (Human), E.coli, N-terminal GST-tagged, 63.0kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human Aldo-keto reductase family 1 member B10 (AKR1B10)

Recombinant Human Aldo-keto reductase family 1 member B10 (AKR1B10) — Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Purity >90%.

SKU: BCRECP-000052 Categories: ,
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Product Specifications

Product SkuBCRECP-000052
Product DescriptionRecombinant Human Aldo-keto reductase family 1 member B10 (AKR1B10) Protein is expressed from E.coli with N-terminal GST-tagged. It contains 1-316aa. [Accession | O60218].
Uniprot No.O60218
Gene NamesAKR1B10
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-316aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal GST-tagged
Molecular weight63.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays strong enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal. Plays a critical role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls).

Biological Context

Subcellular Location: Lysosome; Secreted
Tissue Specificity: Found in many tissues. Highly expressed in small intestine, colon and adrenal gland
Pathway: Cofactor metabolism; retinol metabolism

Product Specifications

Recombinant Human Aldo-keto reductase family 1 member B10 (AKR1B10) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-316aa, with N-terminal GST-tagged tag, molecular weight 63.0kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols (PubMed:12732097, PubMed:18087047, PubMed:19013440, PubMed:19563777, PubMed:9565553). Displays strong enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal (PubMed:12732097, PubMed:18087047). Plays a critical role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls) (PubMed:19013440, PubMed:19563777). Displays no reductase activity towards glucose (PubMed:12732097)

Subcellular Location

Lysosome; Secreted

Tissue Specificity

Found in many tissues. Highly expressed in small intestine, colon and adrenal gland

Pathway

Cofactor metabolism; retinol metabolism

Gene: AKR1B10  |  Organism: Homo sapiens  |  Synonyms: ARL-1; Aldose reductase-like; Aldose reductase-related protein; Small intestine reductase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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