Recombinant Human Biliverdin reductase A (BLVRA), Homo sapiens (Human), E.coli, N-terminal GST-tagged, 60.2kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human Biliverdin reductase A (BLVRA)

Recombinant Human Biliverdin reductase A (BLVRA) — Reduces the gamma-methene bridge of the open tetrapyrrole, biliverdin IXalpha, to bilirubin with the concomitant oxidation of a NADH or NADPH cofactor. Purity >90%.

SKU: BCRECP-000162 Categories: ,
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Product Specifications

Product SkuBCRECP-000162
Product DescriptionRecombinant Human Biliverdin reductase A (BLVRA) Protein is expressed from E.coli with N-terminal GST-tagged. It contains 1-296aa. [Accession | P53004].
Uniprot No.P53004
Gene NamesBLVRA
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-296aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal GST-tagged
Molecular weight60.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Reduces the gamma-methene bridge of the open tetrapyrrole, biliverdin IXalpha, to bilirubin with the concomitant oxidation of a NADH or NADPH cofactor. Does not reduce bilirubin IXbeta. Uses the reactants NADH or NADPH depending on the pH; NADH is used at the acidic pH range (6-6.9) and NADPH at the alkaline range (8.5-8.7).

Biological Context

Subcellular Location: Cytoplasm, cytosol
Tissue Specificity: Liver
Disease Association: Hyperbiliverdinemia (HBLVD) : A condition characterized by a green discoloration of the skin, urine, serum, and other bodily fluids. It is due to increased biliverdin resulting from inefficient conversion to bilirubin. Affected individuals appear to have symptoms only in the context of obstructive cholestasis and/or liver failure. In some cases, green jaundice can resolve after resolution of obstructive cholestasis. [The disease is caused by variants affecting the gene represented in this entry]
Pathway: Porphyrin-containing compound metabolism; protoheme degradation

Product Specifications

Recombinant Human Biliverdin reductase A (BLVRA) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-296aa, with N-terminal GST-tagged tag, molecular weight 60.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Reduces the gamma-methene bridge of the open tetrapyrrole, biliverdin IXalpha, to bilirubin with the concomitant oxidation of a NADH or NADPH cofactor (PubMed:10858451, PubMed:7929092, PubMed:8424666, PubMed:8631357). Does not reduce bilirubin IXbeta (PubMed:10858451). Uses the reactants NADH or NADPH depending on the pH; NADH is used at the acidic pH range (6-6.9) and NADPH at the alkaline range (8.5-8.7) (PubMed:7929092, PubMed:8424666, PubMed:8631357). NADPH, however, is the probable reactant in biological systems (PubMed:7929092)

Subcellular Location

Cytoplasm, cytosol

Disease Association

Hyperbiliverdinemia (HBLVD) : A condition characterized by a green discoloration of the skin, urine, serum, and other bodily fluids. It is due to increased biliverdin resulting from inefficient conversion to bilirubin. Affected individuals appear to have symptoms only in the context of obstructive cholestasis and/or liver failure. In some cases, green jaundice can resolve after resolution of obstructive cholestasis. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Liver

Subunit

Monomer

Pathway

Porphyrin-containing compound metabolism; protoheme degradation

Gene: BLVRA  |  Organism: Homo sapiens  |  Synonyms: Biliverdin-IX alpha-reductase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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