Recombinant Human Biotinidase (BTD), partial, Homo sapiens (Human), E.coli, N-terminal 10xHis-GST-tagged and C-terminal Myc-tagged, 41.3kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human Biotinidase (BTD), partial

Recombinant Human Biotinidase (BTD), partial — recombinant protein from Homo sapiens (Human), expressed in E.coli. Purity >85%.

SKU: BCRECP-000179 Category:
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Product Specifications

Product SkuBCRECP-000179
Product DescriptionRecombinant Human Biotinidase (BTD) Protein is expressed from E.coli with N-terminal 10xHis-GST-tagged and C-terminal Myc-tagged. It contains 322-397aa. [Accession | P43251].
Uniprot No.P43251
Gene NamesBTD
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region322-397aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-GST-tagged and C-terminal Myc-tagged
Molecular weight41.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMetabolism

Function

Catalytic release of biotin from biocytin, the product of biotin-dependent carboxylases degradation.

Biological Context

Subcellular Location: Secreted, extracellular space
Disease Association: Biotinidase deficiency (BTD deficiency) : A juvenile form of multiple carboxylase deficiency, an autosomal recessive disorder of biotin metabolism, characterized by ketoacidosis, hyperammonemia, excretion of abnormal organic acid metabolites, and dermatitis. Biotinidase deficiency is characterized by seizures, hypotonia, skin rash, alopecia, ataxia, hearing loss, and optic atrophy. If untreated, symptoms usually become progressively worse, and coma and death may occur. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Biotinidase (BTD), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 322-397aa, with N-terminal 10xHis-GST-tagged and C-terminal Myc-tagged tag, molecular weight 41.3kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Catalytic release of biotin from biocytin, the product of biotin-dependent carboxylases degradation

Subcellular Location

Secreted, extracellular space

Disease Association

Biotinidase deficiency (BTD deficiency) : A juvenile form of multiple carboxylase deficiency, an autosomal recessive disorder of biotin metabolism, characterized by ketoacidosis, hyperammonemia, excretion of abnormal organic acid metabolites, and dermatitis. Biotinidase deficiency is characterized by seizures, hypotonia, skin rash, alopecia, ataxia, hearing loss, and optic atrophy. If untreated, symptoms usually become progressively worse, and coma and death may occur. [The disease is caused by variants affecting the gene represented in this entry]

Gene: BTD  |  Organism: Homo sapiens
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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