Recombinant Human Hemicentin-1 (HMCN1), partial, Homo sapiens (Human), E.coli, N-terminal 6xHis-tagged, 19.2kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Hemicentin-1 (HMCN1), partial

Recombinant Human Hemicentin-1 (HMCN1), partial — Involved in transforming growth factor beta-mediated rearrangement of the podocyte cytoskeleton which includes reduction of F-actin fibers and broadening, flattening and elongation of podocytes. Purity >90%.

SKU: BCRECP-000838 Category:
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Product Specifications

Product SkuBCRECP-000838
Product DescriptionRecombinant Human Hemicentin-1 (HMCN1) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 4893-5029aa. [Accession | Q96RW7].
Uniprot No.Q96RW7
Gene NamesHMCN1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region4893-5029aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight19.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Involved in transforming growth factor beta-mediated rearrangement of the podocyte cytoskeleton which includes reduction of F-actin fibers and broadening, flattening and elongation of podocytes. Plays a role in basement membrane organization. May promote cleavage furrow maturation during cytokinesis in preimplantation embryos.

Biological Context

Subcellular Location: Secreted, extracellular space, extracellular matrix, basement membrane; Cytoplasm; Cell junction; Cleavage furrow
Tissue Specificity: Expressed in skin fibroblasts and retinal pigment epithelium (RPE) cells
Disease Association: Macular degeneration, age-related, 1 (ARMD1) : A form of age-related macular degeneration, a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Hemicentin-1 (HMCN1), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 4893-5029aa, with N-terminal 6xHis-tagged tag, molecular weight 19.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Involved in transforming growth factor beta-mediated rearrangement of the podocyte cytoskeleton which includes reduction of F-actin fibers and broadening, flattening and elongation of podocytes (PubMed:29488390). Plays a role in basement membrane organization (By similarity). May promote cleavage furrow maturation during cytokinesis in preimplantation embryos (By similarity). May play a role in the architecture of adhesive and flexible epithelial cell junctions (By similarity). May play a role during myocardial remodeling by imparting an effect on cardiac fibroblast migration (By similarity)

Subcellular Location

Secreted, extracellular space, extracellular matrix, basement membrane; Cytoplasm; Cell junction; Cleavage furrow

Disease Association

Macular degeneration, age-related, 1 (ARMD1) : A form of age-related macular degeneration, a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Expressed in skin fibroblasts and retinal pigment epithelium (RPE) cells

Gene: HMCN1  |  Organism: Homo sapiens  |  Synonyms: Fibulin-6
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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