Recombinant Human Interleukin-10 receptor subunit alpha (IL10RA)-VLPs (Active), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged, 62.2 kDa, purity >/
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Recombinant Human Interleukin-10 receptor subunit alpha (IL10RA)-VLPs (Active)

Recombinant Human Interleukin-10 receptor subunit alpha (IL10RA)-VLPs (Active) — Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Purity /.

SKU: BCACP-000661 Categories: , ,
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Product SKUBCACP-000661
Product DescriptionRecombinant Human Interleukin-10 receptor subunit alpha (IL10RA)-VLPs protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 22-578aa. [Accession | Q13651].
Uniprot No.Q13651
Gene NamesIL10RA
Purity/
Expression SystemMammalian cell
Expression Region22-578aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged
Molecular weight62.2 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis CLEC7A at 2 μg/mL can bind Anti-CLEC7A recombinant antibody . The EC50 is 2.544-3.020 ng/mL.;CLEC7A Recombinant Monoclonal Antibody captured on Protein A Chip can bind Recombinant Macaca fascicularis CLEC7A with an affinity constant of 0.537 nM as detected by MetaSPR Assay (WeSPRTM 200).
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesInterleukin-10 receptor subunit alpha; IL-10 receptor subunit alpha; IL-10R subunit alpha; IL-10RA; CDw210a; Interleukin-10 receptor subunit 1; IL-10R subunit 1; IL-10R1; CD antigen CD210

Function

Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well. In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively.

Biological Context

Subcellular Location: Cell membrane (Single-pass type I membrane protein); Cytoplasm
Tissue Specificity: Primarily expressed in hematopoetic cells including B-cells, T-cells, NK cells, monocytes and macrophages. Not expressed in non-hematopoetic cells such as fibroblasts or endothelial cells
Disease Association: Inflammatory bowel disease 28, autosomal recessive (IBD28) : A chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Interleukin-10 receptor subunit alpha (IL10RA)-VLPs (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 22-578aa, with C-terminal 10xHis-tagged tag, molecular weight 62.2 kDa, purity /. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis CLEC7A at 2 μg/mL can bind Anti-CLEC7A recombinant antibody . The EC50 is 2.544-3.020 ng/mL.;CLEC7A Recombinant Monoclonal Antibody captured on Protein A Chip can bind Recombinant Macaca fascicularis CLEC7A with an affinity constant of 0.537 nM as detected by MetaSPR Assay (WeSPRTM 200).

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well (PubMed:16982608). In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively (PubMed:12133952). These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy (PubMed:26962683)

Subcellular Location

Cell membrane (Single-pass type I membrane protein); Cytoplasm

Disease Association

Inflammatory bowel disease 28, autosomal recessive (IBD28) : A chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Primarily expressed in hematopoetic cells including B-cells, T-cells, NK cells, monocytes and macrophages. Not expressed in non-hematopoetic cells such as fibroblasts or endothelial cells

Subunit

(Microbial infection) Interacts with Epstein-Barr virus protein IL10

Gene: IL10RA  |  Organism: Homo sapiens  |  Synonyms: CDw210a; Interleukin-10 receptor subunit 1

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