Recombinant Human Retinol dehydrogenase 12 (RDH12), Homo sapiens (Human), E.coli, N-terminal 6xHis-SUMO-tagged, 51.1kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human Retinol dehydrogenase 12 (RDH12)

Recombinant Human Retinol dehydrogenase 12 (RDH12) — Retinoids dehydrogenase/reductase with a clear preference for NADP. Purity >90%.

SKU: BCRECP-001528 Categories: ,
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Product Specifications

Product SkuBCRECP-001528
Product DescriptionRecombinant Human Retinol dehydrogenase 12 (RDH12) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 1-316aa. [Accession | Q96NR8].
Uniprot No.Q96NR8
Gene NamesRDH12
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-316aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight51.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMetabolism

Function

Retinoids dehydrogenase/reductase with a clear preference for NADP. Displays high activity towards 9-cis, 11-cis and all-trans-retinal. Shows very weak activity towards 13-cis-retinol.

Biological Context

Subcellular Location: Endoplasmic reticulum membrane
Tissue Specificity: Widely expressed, mostly in retina, kidney, brain, skeletal muscle, pancreas and stomach
Disease Association: Leber congenital amaurosis 13 (LCA13) : A severe dystrophy of the retina, typically becoming evident in the first years of life. Visual function is usually poor and often accompanied by nystagmus, sluggish or near-absent pupillary responses, photophobia, high hyperopia and keratoconus. [The disease is caused by variants affecting the gene represented in this entry] | Retinitis pigmentosa 53 (RP53) : A retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP53 inheritance is autosomal dominant or autosomal recessive. [The disease is caused by variants affecting the gene represented in this entry]
Pathway: Cofactor metabolism; retinol metabolism

Product Specifications

Recombinant Human Retinol dehydrogenase 12 (RDH12) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-316aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 51.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Retinoids dehydrogenase/reductase with a clear preference for NADP. Displays high activity towards 9-cis, 11-cis and all-trans-retinal. Shows very weak activity towards 13-cis-retinol (PubMed:12226107, PubMed:15865448). Also exhibits activity, albeit with lower affinity than for retinaldehydes, towards lipid peroxidation products (C9 aldehydes) such as 4-hydroxynonenal and trans-2-nonenal (PubMed:15865448, PubMed:19686838). May play an important function in photoreceptor cells to detoxify 4-hydroxynonenal and potentially other toxic aldehyde products resulting from lipid peroxidation (PubMed:19686838). Has no dehydrogenase activity towards steroids (PubMed:12226107, PubMed:15865448)

Subcellular Location

Endoplasmic reticulum membrane

Disease Association

Leber congenital amaurosis 13 (LCA13) : A severe dystrophy of the retina, typically becoming evident in the first years of life. Visual function is usually poor and often accompanied by nystagmus, sluggish or near-absent pupillary responses, photophobia, high hyperopia and keratoconus. [The disease is caused by variants affecting the gene represented in this entry] | Retinitis pigmentosa 53 (RP53) : A retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP53 inheritance is autosomal dominant or autosomal recessive. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Widely expressed, mostly in retina, kidney, brain, skeletal muscle, pancreas and stomach

Pathway

Cofactor metabolism; retinol metabolism

Gene: RDH12  |  Organism: Homo sapiens  |  Synonyms: All-trans and 9-cis retinol dehydrogenase; Short chain dehydrogenase/reductase family 7C member 2
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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