Recombinant Human Serine/threonine-protein kinase PLK2 (PLK2)

Function

Tumor suppressor serine/threonine-protein kinase involved in synaptic plasticity, centriole duplication and G1/S phase transition. Polo-like kinases act by binding and phosphorylating proteins that are already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates CPAP, NPM1, RAPGEF2, RASGRF1, SNCA, SIPA1L1 and SYNGAP1. Plays a key role in synaptic plasticity and memory by regulating the Ras and Rap protein signaling: required for overactivity-dependent spine remodeling by phosphorylating the Ras activator RASGRF1 and the Rap inhibitor SIPA1L1 leading to their degradation by the proteasome. Conversely, phosphorylates the Rap activator RAPGEF2 and the Ras inhibitor SYNGAP1, promoting their activity. Also regulates synaptic plasticity independently of kinase activity, via its interaction with NSF that disrupts the interaction between NSF and the GRIA2 subunit of AMPARs, leading to a rapid rundown of AMPAR-mediated current that occludes long term depression. Required for procentriole formation and centriole duplication by phosphorylating CPAP and NPM1, respectively. Its induction by p53/TP53 suggests that it may participate in the mitotic checkpoint following stress

Subcellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole; Cell projection, dendrite

Tissue Specificity

Expressed at higher level in the fetal lung, kidney, spleen and heart

Subunit

Interacts with NSF; causing NSF dissociation from GRIA2. Interacts with CIB1 (By similarity)

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• Anderson K.M. et al. Identification and characterization of human serum-inducible kinase (SNK), a novel member of the polo-kinase family of cell cycle regulators: potential implication for regulation of vascular smooth muscle proliferation. (JAN-)
• Schmutz J. et al. The DNA sequence and comparative analysis of human chromosome 5. — Nature (2004)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)
• Fidler C. et al. PubMed: CI-8N9D4I9H35KOQ (JAN-)
• Shimizu-Yoshida Y. et al. Radiation-inducible hSNK gene is transcriptionally regulated by p53 binding homology element in human thyroid cells. — Biochem. Biophys. Res. Commun. (2001)
• Warnke S. et al. Polo-like kinase-2 is required for centriole duplication in mammalian cells. — Curr. Biol. (2004)
• Syed N. et al. Transcriptional silencing of Polo-like kinase 2 (SNK/PLK2) is a frequent event in B-cell malignancies. — Blood (2006)