Recombinant Human Sphingomyelin phosphodiesterase 3 (SMPD3), partial, Homo sapiens (Human), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 68.8kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human Sphingomyelin phosphodiesterase 3 (SMPD3), partial

Recombinant Human Sphingomyelin phosphodiesterase 3 (SMPD3), partial — Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Purity >90%.

SKU: BCRECP-001669 Categories: ,
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Product Specifications

Product SkuBCRECP-001669
Product DescriptionRecombinant Human Sphingomyelin phosphodiesterase 3 (SMPD3) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 86-655aa. [Accession | Q9NY59].
Uniprot No.Q9NY59
Gene NamesSMPD3
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region86-655aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight68.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasNeuroscience

Function

Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine.

Biological Context

Subcellular Location: Golgi apparatus membrane (Lipid-anchor); Cell membrane (Lipid-anchor)
Tissue Specificity: Predominantly expressed in brain
Pathway: Lipid metabolism; sphingolipid metabolism

Product Specifications

Recombinant Human Sphingomyelin phosphodiesterase 3 (SMPD3), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 86-655aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 68.8kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization (PubMed:10823942, PubMed:14741383, PubMed:15051724). Binds to anionic phospholipids (APLs) such as phosphatidylserine (PS) and phosphatidic acid (PA) that modulate enzymatic activity and subcellular location. May be involved in IL-1-beta-induced JNK activation in hepatocytes (By similarity). May act as a mediator in transcriptional regulation of NOS2/iNOS via the NF-kappa-B activation under inflammatory conditions (By similarity)

Subcellular Location

Golgi apparatus membrane (Lipid-anchor); Cell membrane (Lipid-anchor)

Tissue Specificity

Predominantly expressed in brain

Pathway

Lipid metabolism; sphingolipid metabolism

Gene: SMPD3  |  Organism: Homo sapiens  |  Synonyms: Neutral sphingomyelinase 2; Neutral sphingomyelinase II
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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