Recombinant Lassa virus RING finger protein Z (Z), Lassa virus (strain Mouse/Sierra Leone/Josiah/1976) (LASV), E.coli, N-terminal 6xHis-tagged, 14.6kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Lassa virus RING finger protein Z (Z)

Recombinant Lassa virus RING finger protein Z (Z) — Plays a crucial role in virion assembly and budding. Purity >90%.

SKU: BCRECP-004780 Categories: ,
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Product Specifications

Product SkuBCRECP-004780
Product DescriptionRecombinant Lassa virus RING finger protein Z (Z) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 2-99aa. [Accession | O73557].
Uniprot No.O73557
Gene NamesZ
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-99aa
SpeciesLassa virus (strain Mouse/Sierra Leone/Josiah/1976) (LASV)
Tag InfoN-terminal 6xHis-tagged
Molecular weight14.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Plays a crucial role in virion assembly and budding. Expressed late in the virus life cycle, it acts as an inhibitor of viral transcription and RNA synthesis by interacting with the viral polymerase L. Presumably recruits the NP encapsidated genome to cellular membranes at budding sites via direct interaction with NP.

Biological Context

Subcellular Location: Virion; Host cytoplasm, host perinuclear region; Host cell membrane (Lipid-anchor)

Product Specifications

Recombinant Lassa virus RING finger protein Z (Z) is a recombinant protein from Lassa virus (strain Mouse/Sierra Leone/Josiah/1976) (LASV), expressed in E.coli, covering amino acids 2-99aa, with N-terminal 6xHis-tagged tag, molecular weight 14.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Plays a crucial role in virion assembly and budding. Expressed late in the virus life cycle, it acts as an inhibitor of viral transcription and RNA synthesis by interacting with the viral polymerase L. Presumably recruits the NP encapsidated genome to cellular membranes at budding sites via direct interaction with NP. Plays critical roles in the final steps of viral release by interacting with host TSG101, a member of the vacuolar protein-sorting pathway and using other cellular host proteins involved in vesicle formation pathway. The budding of the virus progeny occurs after association of protein Z with the viral glycoprotein complex SSP-GP1-GP2 at the cell periphery, step that requires myristoylation of protein Z. Also selectively represses protein production by associating with host eIF4E(PubMed:12970458, PubMed:14990716). In cell-based minigenome assay, has an inhibitory effect on the ribonucleoprotein machinery (vRNP), which is responsible for the replication and transcription of the viral genome (By similarity). Myristoylation and oligomerization of protein Z are not required for its inhibitory vRNP activity, but myristoylation probably plays a role in assembly and budding (By similarity)

Subcellular Location

Virion; Host cytoplasm, host perinuclear region; Host cell membrane (Lipid-anchor)

Subunit

Monomer (PubMed:26912609). Homododecamer; ring-like structure (PubMed:26912609). Interacts with protein NP; this interaction probably directs the encapsidated genome to budding sites (By similarity). Interacts (via RING domain) with polymerase L; this interaction inhibits viral transcription and replication, Z partially blocks the product exit tunnel for the releasing nascent RNA product. Interacts with the glycoprotein complex; this interaction plays a role in virion budding. Interacts with host eIF4E; this interaction results in eIF4E reduced affinity for its substrate, the 5'-m7 G cap structure. Interacts (via late-budding domain) with host TSG101; this interaction is essential for budding and release of viral particles. Interacts with host RPLP0; this interaction may serve to load ribosome-like particles inside the virion. Interacts with host PML; this interaction induces PML bodies redistribution in the cytoplasm upon viral infection. Interacts with host TAX1BP1 (PubMed:30909570)

Gene: Z  |  Organism: Lassa virus (strain Mouse/Sierra Leone/Josiah/1976)  |  Synonyms: Zinc-binding protein
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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