Recombinant Mouse Golgin subfamily A member 2 (Golga2), partial, Mus musculus (Mouse), E.coli, C-terminal 6xHis-tagged, 29.7kDa, purity >95% as determined by SDS-PAGE.

Recombinant Mouse Golgin subfamily A member 2 (Golga2), partial

Recombinant Mouse Golgin subfamily A member 2 (Golga2), partial — Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion t… Purity >95%.

SKU: BCRECP-002884 Category:
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Product Specifications

Product SkuBCRECP-002884
Product DescriptionRecombinant Mouse Golgin subfamily A member 2 (Golga2) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 800-999aa. [Accession | Q921M4].
Uniprot No.Q921M4
Gene NamesGolga2
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region800-999aa
SpeciesMus musculus (Mouse)
Tag InfoC-terminal 6xHis-tagged
Molecular weight29.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane. Required for normal protein transport from the endoplasmic reticulum to the Golgi apparatus and the cell membrane. Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus.

Biological Context

Subcellular Location: Golgi apparatus, cis-Golgi network membrane (Peripheral membrane protein); Endoplasmic reticulum-Golgi intermediate compartment membrane (Peripheral membrane protein); Cytoplasm, cytoskeleton, spindle pole
Tissue Specificity: Widely expressed. Detected in brain, kidney, lung, liver, spleen, heart, skeletal muscle, thymus and pancreas. Detected in spermatocytes. Present in oocytes during all oocyte meiotic maturation (at protein level)

Product Specifications

Recombinant Mouse Golgin subfamily A member 2 (Golga2), partial is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 800-999aa, with C-terminal 6xHis-tagged tag, molecular weight 29.7kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane (PubMed:28028212). Required for normal protein transport from the endoplasmic reticulum to the Golgi apparatus and the cell membrane (PubMed:28028212). Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis. Also plays a key role in spindle pole assembly and centrosome organization (By similarity). Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin-alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle (By similarity). Regulates the meiotic spindle pole assembly, probably via the same mechanism (PubMed:21552007). Also regulates the centrosome organization (By similarity). Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins (By similarity)

Subcellular Location

Golgi apparatus, cis-Golgi network membrane (Peripheral membrane protein); Endoplasmic reticulum-Golgi intermediate compartment membrane (Peripheral membrane protein); Cytoplasm, cytoskeleton, spindle pole

Tissue Specificity

Widely expressed. Detected in brain, kidney, lung, liver, spleen, heart, skeletal muscle, thymus and pancreas (PubMed:26742501, PubMed:28028212). Detected in spermatocytes (PubMed:28617811). Present in oocytes during all oocyte meiotic maturation (at protein level)

Subunit

Homodimer, may assemble into homohexamers (By similarity). Homotetramer; forms a parallel homotetramer with a flexible rod-like structure that can give rise to I- and Y-shaped conformations. Interacts with GORASP1/GRASP65. The homooligomer forms a complex with GORASP1 with a 1:1 stoichiometry (By similarity). Interacts with RAB1B that has been activated by GTP-binding. Interacts with p115/USO1; interaction with p115/USO1 inhibits interaction with STX5 and/or RAB1B. Interacts with STX5 (By similarity). Interacts with ZFPL1 (By similarity). Interacts with AKAP450/AKAP9; leading to recruit AKAP450/AKAP9 to the cis-Golgi (By similarity)

Gene: Golga2  |  Organism: Mus musculus  |  Synonyms: 130 kDa cis-Golgi matrix protein
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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