Recombinant Mouse Regenerating islet-derived protein 3-gamma (Reg3g), partial, Mus musculus (Mouse), E.coli, C-terminal 6xHis-tagged, 23.3kDa, purity >95% as determined by SDS-PAGE.

Recombinant Mouse Regenerating islet-derived protein 3-gamma (Reg3g), partial

Recombinant Mouse Regenerating islet-derived protein 3-gamma (Reg3g), partial — Has bactericidal activity against L. Purity >95%.

SKU: BCRECP-004250 Category:
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Product Specifications

Product SkuBCRECP-004250
Product DescriptionRecombinant Mouse Regenerating islet-derived protein 3-gamma (Reg3g) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 27-174aa. [Accession | O09049].
Uniprot No.O09049
Gene NamesReg3g
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region27-174aa
SpeciesMus musculus (Mouse)
Tag InfoC-terminal 6xHis-tagged
Molecular weight23.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasImmunology

Function

Has bactericidal activity against L.monocytogenes and methicillin-resistant S.aureus.

Biological Context

Subcellular Location: Secreted; Cytoplasm
Tissue Specificity: Predominantly expressed in the small intestine, including Paneth cells (at protein level). Hardly detectable in the colon (at protein level). Highly expressed in the lung epithelium during methicillin-resistant S.aureus infection and allergic airway inflammation (at protein level). Skin injury increases its epidermal expression. Also expressed in the pancreas. Expressed by nocireceptors

Product Specifications

Recombinant Mouse Regenerating islet-derived protein 3-gamma (Reg3g), partial is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 27-174aa, with C-terminal 6xHis-tagged tag, molecular weight 23.3kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Has bactericidal activity against L.monocytogenes and methicillin-resistant S.aureus

Subcellular Location

Secreted; Cytoplasm

Tissue Specificity

Predominantly expressed in the small intestine, including Paneth cells (at protein level). Hardly detectable in the colon (at protein level) (PubMed:16504538, PubMed:16931762, PubMed:17635956, PubMed:21998396). Highly expressed in the lung epithelium during methicillin-resistant S.aureus infection and allergic airway inflammation (at protein level) (PubMed:28811323). Skin injury increases its epidermal expression (PubMed:22727489, PubMed:23401489, PubMed:27830702). Also expressed in the pancreas (PubMed:9055810). Expressed by nocireceptors (PubMed:35263589)

Subunit

Forms a hexameric membrane-permeabilizing oligomeric pore on membrane phospholipids. The hexamer is formed by three dimers related by helical symmetry. Forms filaments, filamentation traps pore complexes and limits damage to host cells. Interacts with EXTL3

Gene: Reg3g  |  Organism: Mus musculus  |  Synonyms: Pancreatitis-associated protein 3; Regenerating islet-derived protein III-gamma
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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