Recombinant Murine polyomavirus Minor capsid protein VP2, Murine polyomavirus (strain Kilham) (MPyV) (Murine pneumotropic virus), E.coli, N-terminal 6xHis-tagged, 41.4kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Murine polyomavirus Minor capsid protein VP2

Recombinant Murine polyomavirus Minor capsid protein VP2 — Structural protein that resides within the core of the capsid surrounded by 72 VP1 pentamers. Purity >85%.

SKU: BCRECP-003627 Categories: , ,
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Product Specifications

Product SkuBCRECP-003627
Product DescriptionRecombinant Murine polyomavirus Minor capsid protein VP2 Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 2-324aa. [Accession | P24596].
Uniprot No.P24596
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-324aa
SpeciesMurine polyomavirus (strain Kilham) (MPyV) (Murine pneumotropic virus)
Tag InfoN-terminal 6xHis-tagged
Molecular weight41.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research Areasothers

Function

Structural protein that resides within the core of the capsid surrounded by 72 VP1 pentamers. Following virus endocytosis and trafficking to the endoplasmic reticulum, VP2 and VP3 form oligomers and integrate into the endoplasmic reticulum membrane. Heterooligomer VP2-VP3 may create a viroporin for transporting the viral genome across the endoplasmic reticulum membrane to the cytoplasm.

Biological Context

Subcellular Location: Virion; Host nucleus; Host endoplasmic reticulum; Host endoplasmic reticulum membrane

Product Specifications

Recombinant Murine polyomavirus Minor capsid protein VP2 is a recombinant protein from Murine polyomavirus (strain Kilham) (MPyV) (Murine pneumotropic virus), expressed in E.coli, covering amino acids 2-324aa, with N-terminal 6xHis-tagged tag, molecular weight 41.4kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Structural protein that resides within the core of the capsid surrounded by 72 VP1 pentamers. Following virus endocytosis and trafficking to the endoplasmic reticulum, VP2 and VP3 form oligomers and integrate into the endoplasmic reticulum membrane. Heterooligomer VP2-VP3 may create a viroporin for transporting the viral genome across the endoplasmic reticulum membrane to the cytoplasm. Nuclear entry of the viral DNA involves the selective exposure and importin recognition of VP2 or VP3 nuclear localization signal (shared C-terminus). Plays a role in virion assembly within the nucleus (By similarity)

Subcellular Location

Virion; Host nucleus; Host endoplasmic reticulum; Host endoplasmic reticulum membrane

Subunit

Interacts (via D1 domain) with VP1

Organism: Murine polyomavirus (strain Kilham)  |  Synonyms: Minor structural protein VP2

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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