Recombinant Porcine reproductive and respiratory syndrome virus Glycoprotein 5 (GP5), partial, Porcine reproductive and respiratory syndrome virus (isolate Pig/United States/SD 01-08/2001) (PRRSV), E.coli, N-terminal GST-tagged, 30.2kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Porcine reproductive and respiratory syndrome virus Glycoprotein 5 (GP5), partial

Recombinant Porcine reproductive and respiratory syndrome virus Glycoprotein 5 (GP5), partial — Major envelope protein present in abundant amounts in the virion envelope. Purity >95%.

SKU: BCRECP-002892 Categories: ,
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Product Specifications

Product SkuBCRECP-002892
Product DescriptionRecombinant Porcine reproductive and respiratory syndrome virus Glycoprotein 5 (GP5) Protein is expressed from E.coli with N-terminal GST-tagged. It contains 33-63aa. [Accession | A0MD34].
Uniprot No.A0MD34
Gene NamesGP5
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region33-63aa
SpeciesPorcine reproductive and respiratory syndrome virus (isolate Pig/United States/SD 01-08/2001) (PRRSV)
Tag InfoN-terminal GST-tagged
Molecular weight30.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Major envelope protein present in abundant amounts in the virion envelope. Mediates virion sialic acid-dependent attachment the sialoadhesin receptor SIGLEC1. This attachment induces virion internalization into alveolar macrophages predominantly through clathrin-dependent endocytosis.

Biological Context

Subcellular Location: Virion membrane (Multi-pass membrane protein)

Product Specifications

Recombinant Porcine reproductive and respiratory syndrome virus Glycoprotein 5 (GP5), partial is a recombinant protein from Porcine reproductive and respiratory syndrome virus (isolate Pig/United States/SD 01-08/2001) (PRRSV), expressed in E.coli, covering amino acids 33-63aa, with N-terminal GST-tagged tag, molecular weight 30.2kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Major envelope protein present in abundant amounts in the virion envelope. Mediates virion sialic acid-dependent attachment the sialoadhesin receptor SIGLEC1. This attachment induces virion internalization into alveolar macrophages predominantly through clathrin-dependent endocytosis

Subcellular Location

Virion membrane (Multi-pass membrane protein)

Subunit

Heterodimer with the membrane protein; disulfide-linked. This heterodimerization is required for transport to the Golgi complex (By similarity). Interacts with host SIGLEC1; this interaction plays a role in virus entry into host cell (By similarity). Interacts with glycoprotein 4

Gene: GP5  |  Organism: Porcine reproductive and respiratory syndrome virus (isolate Pig/United States/SD 01-08/2001)  |  Synonyms: G(L)

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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