Recombinant Rat Serotransferrin (Tf), Rattus norvegicus (Rat), E.coli, N-terminal 6xHis-KSI-tagged, 89.9kDa, purity >85% as determined by SDS-PAGE.

Recombinant Rat Serotransferrin (Tf)

Recombinant Rat Serotransferrin (Tf) — Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Purity >85%.

SKU: BCRECP-004543 Category:
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Product Specifications

Product SkuBCRECP-004543
Product DescriptionRecombinant Rat Serotransferrin (Tf) Protein is expressed from E.coli with N-terminal 6xHis-KSI-tagged. It contains 20-698aa. [Accession | P12346].
Uniprot No.P12346
Gene NamesTf
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region20-698aa
SpeciesRattus norvegicus (Rat)
Tag InfoN-terminal 6xHis-KSI-tagged
Molecular weight89.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasEpigenetics and Nuclear Signaling

Function

Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Expressed by the liver and secreted in plasma

Product Specifications

Recombinant Rat Serotransferrin (Tf) is a recombinant protein from Rattus norvegicus (Rat), expressed in E.coli, covering amino acids 20-698aa, with N-terminal 6xHis-KSI-tagged tag, molecular weight 89.9kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation

Subcellular Location

Secreted

Tissue Specificity

Expressed by the liver and secreted in plasma

Subunit

Monomer. Part of a complex composed of SLC40A1/ferroportin, TF/transferrin and HEPH/hephaestin that transfers iron from cells to transferrin

Gene: Tf  |  Organism: Rattus norvegicus  |  Synonyms: Beta-1 metal-binding globulin; Liver regeneration-related protein LRRG03; Siderophilin
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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