Recombinant Tick-borne encephalitis virus Far Eastern subtype Genome polyprotein, partial, Tick-borne encephalitis virus Far Eastern subtype (strain Sofjin) (SOFV) (Sofjin virus), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 56.2kDa, purity >85% as determined by SDS-PAGE.
,

Recombinant Tick-borne encephalitis virus Far Eastern subtype Genome polyprotein, partial

Recombinant Tick-borne encephalitis virus Far Eastern subtype Genome polyprotein, partial — Replicates the viral (+) and (-) genome, and performs the capping of genomes in the cytoplasm. Purity >85%.

SKU: BCRECP-003666 Categories: ,
Online Inquiry →

Product Specifications

Product SkuBCRECP-003666
Product DescriptionRecombinant Tick-borne encephalitis virus Far Eastern subtype Genome polyprotein, partial Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 281-727aa. [Accession | P07720].
Uniprot No.P07720
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region281-727aa
SpeciesTick-borne encephalitis virus Far Eastern subtype (strain Sofjin) (SOFV) (Sofjin virus)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight56.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Replicates the viral (+) and (-) genome, and performs the capping of genomes in the cytoplasm. NS5 methylates viral RNA cap at guanine N-7 and ribose 2'-O positions. Besides its role in RNA genome replication, also prevents the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) signaling pathway.

Biological Context

Subcellular Location: Host endoplasmic reticulum membrane (Peripheral membrane protein); Host nucleus

Product Specifications

Recombinant Tick-borne encephalitis virus Far Eastern subtype Genome polyprotein, partial is a recombinant protein from Tick-borne encephalitis virus Far Eastern subtype (strain Sofjin) (SOFV) (Sofjin virus), expressed in E.coli, covering amino acids 281-727aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 56.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Replicates the viral (+) and (-) genome, and performs the capping of genomes in the cytoplasm. NS5 methylates viral RNA cap at guanine N-7 and ribose 2'-O positions. Besides its role in RNA genome replication, also prevents the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) signaling pathway. Inhibits host TYK2 and STAT2 phosphorylation, thereby preventing activation of JAK-STAT signaling pathway

Subcellular Location

Host endoplasmic reticulum membrane (Peripheral membrane protein); Host nucleus

Subunit

Homodimer (By similarity). Interacts with host STAT2; this interaction inhibits the phosphorylation of the latter, and, when all viral proteins are present (polyprotein), targets STAT2 for degradation (By similarity). Interacts with serine protease NS3 (By similarity). Interacts (via C-terminus) with host SCRIB (via PDZ3); this interaction targets NS5 to the cell membrane periphery and nucleus, thereby allowing efficient host nuclear STAT1 inhibition (By similarity)

Organism: Tick-borne encephalitis virus Far Eastern subtype (strain Sofjin)
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top