Recombinant Staphylococcus aureus Enterotoxin type C-3 (entC3), Staphylococcus aureus, E.coli, N-terminal 6xHis-SUMO-tagged, 43.6kDa, purity >90% as determined by SDS-PAGE.

Recombinant Staphylococcus aureus Enterotoxin type C-3 (entC3)

Recombinant Staphylococcus aureus Enterotoxin type C-3 (entC3) — Staphylococcal enterotoxin that activates the host immune system by binding as unprocessed molecules to major histocompatibility (MHC) complex class II and T-cell receptor (TCR) molecules. Purity >90%.

SKU: BCRECP-002617 Category:
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Product Specifications

Product SkuBCRECP-002617
Product DescriptionRecombinant Staphylococcus aureus Enterotoxin type C-3 (entC3) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 28-266aa. [Accession | P0A0L5].
Uniprot No.P0A0L5
Gene NamesentC3
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region28-266aa
SpeciesStaphylococcus aureus
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight43.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Staphylococcal enterotoxin that activates the host immune system by binding as unprocessed molecules to major histocompatibility (MHC) complex class II and T-cell receptor (TCR) molecules. In turn, this ternary complex activates a large number of T-lymphocytes initiating a systemic release of pro-inflammatory cytokines.

Product Specifications

Recombinant Staphylococcus aureus Enterotoxin type C-3 (entC3) — Staphylococcal enterotoxin that activates the host immune system by binding as unprocessed molecules to major histocompatibility (MHC) complex class II and T-cell receptor (TCR) molecules. Purity >90%. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Staphylococcal enterotoxin that activates the host immune system by binding as unprocessed molecules to major histocompatibility (MHC) complex class II and T-cell receptor (TCR) molecules. In turn, this ternary complex activates a large number of T-lymphocytes initiating a systemic release of pro-inflammatory cytokines (PubMed:10229190, PubMed:28849041). Also causes the intoxication staphylococcal food poisoning syndrome (By similarity)

Subcellular Location

Secreted

Subunit

Interacts with MHC class II molecules composed of alpha/HLA-DRA and beta/HLA-DRB1 chains (PubMed:10229190). Interacts with host T-cell receptor/TCR beta variable chain TRBV8-2 (PubMed:10229190, PubMed:20836565)

Gene: entC3  |  Organism: Staphylococcus aureus  |  Synonyms: SEC3
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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