Recombinant Bovine Ferrochelatase, mitochondrial (FECH), Bos taurus (Bovine), E.coli, N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged, 58.1kDa, purity >85% as determined by SDS-PAGE.

Recombinant Bovine Ferrochelatase, mitochondrial (FECH)

Recombinant Bovine Ferrochelatase, mitochondrial (FECH) — recombinant protein from Bos taurus (Bovine), expressed in E.coli. Purity >85%.

SKU: BCRECP-002720 Category:
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Product Specifications

Product SkuBCRECP-002720
Product DescriptionRecombinant Bovine Ferrochelatase, mitochondrial (FECH) Protein is expressed from E.coli with N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged. It contains 48-416aa. [Accession | P22600].
Uniprot No.P22600
Gene NamesFECH
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region48-416aa
SpeciesBos taurus (Bovine)
Tag InfoN-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Molecular weight58.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCardiovascular

Function

Catalyzes the ferrous insertion into protoporphyrin IX and participates in the terminal step in the heme biosynthetic pathway.

Biological Context

Subcellular Location: Mitochondrion inner membrane (Peripheral membrane protein)
Pathway: Porphyrin-containing compound metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1

Product Specifications

Recombinant Bovine Ferrochelatase, mitochondrial (FECH) is a recombinant protein from Bos taurus (Bovine), expressed in E.coli, covering amino acids 48-416aa, with N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged tag, molecular weight 58.1kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Catalyzes the ferrous insertion into protoporphyrin IX and participates in the terminal step in the heme biosynthetic pathway

Subcellular Location

Mitochondrion inner membrane (Peripheral membrane protein)

Subunit

Homodimer. Homotetramer (By similarity). Interaction with PGRMC1; the interaction results in decreased FECH activity. Interacts with ABCB10 and SLC25A37; this interaction forms an oligomeric complex. Forms a complex with ABCB7 and ABCB10, where a dimeric FECH bridges ABCB7 and ABCB10 homodimers; this complex may be required for cellular iron homeostasis, mitochondrial function and heme biosynthesis. Interacts with ABCB7 and ABCB10 (By similarity). Interacts with MIMS2 and PPOX to form an oligomeric iron transport complex required for heme synthesis (By similarity)

Pathway

Porphyrin-containing compound metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1

Gene: FECH  |  Organism: Bos taurus  |  Synonyms: Heme synthase; Protoheme ferro-lyase

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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